Abstract：
Wastewater-Based Epidemiology (WBE) is widely used to monitor the progression of SARS-CoV-2 pandemic. While there is a clear correlation between the number of COVID patients in a sewershed and the viral load in the wastewater, there is notable variability across different treatment plants. In particular, some facilities consistently exhibit higher viral content per diagnosed patient, implying a potential underestimation of the number of COVID patients, while others show a low viral load per diagnosed case, indicating potential attenuation of genetic material from the sewershed. In this study, we investigated the impact of nonylphenol ethoxylate (NPHE), linear alkylbenzene sulfonic acid (LABS), bisoctyl dimethyl ammonium chloride (BDAC), and didecyldimethylammonium chloride (DDAC), the surfactants that have been commonly used as detergents, emulsifiers, wetting agents on the stability of SARS-CoV-2 in wastewater. The results showed multiple and dynamic mechanisms, including degradation and desorption, can occur simultaneously during the interaction between SARS-CoV-2 and different chemicals depending on the physicochemical properties of each chemical. Through the elucidation of the dynamic interactions, the findings from this study could help the state health organizations and scientific community to optimize the SARS-CoV-2 wastewater-based epidemiology strategies.
摘要：
基于废水的流行病学（WBE）被广泛用于监测SARS-CoV-2大流行的进展。尽管下水道中的COVID患者数量与废水中的病毒载量之间存在明显的相关性，不同处理厂之间存在明显的差异。特别是,一些设施始终显示每个确诊患者的病毒含量较高，这意味着可能低估了COVID患者的数量，而其他人则显示每个诊断病例的病毒载量较低，表明下水道遗传物质的潜在衰减。在这项研究中,我们研究了壬基酚乙氧基化物(NPHE)的影响，直链烷基苯磺酸（LABS），二辛基二甲基氯化铵（BDAC），和二癸基二甲基氯化铵（DDAC），通常用作洗涤剂的表面活性剂，乳化剂,润湿剂对SARS-CoV-2废水稳定性的影响。结果显示了多种动态机制，包括降解和解吸，可以在SARS-CoV-2与不同化学物质之间的相互作用过程中同时发生，具体取决于每种化学物质的物理化学性质。通过对动态相互作用的阐明，这项研究的结果可以帮助国家卫生组织和科学界优化SARS-CoV-2废水流行病学策略。
