PDF(Pubmed)
Abstract：
UNASSIGNED: Ischemia-reperfusion (IR) injury to a part of the body can cause damage to distant organs such as the kidney and heart. This study investigated the protective effects of safranal against IR-induced renal injury.
UNASSIGNED: Used in this study were 24 Wistar Albino male rats, which were divided into 3 equal and randomised groups. The sham group underwent laparotomy only. In the IR group, the infrarenal aorta was clamped for 1 h, and then reperfused for 2 h. In the IR-safranal group, safranal was administered 30 min before the procedure and IR injury was induced in the same way as in the IR group. After the procedure, blood and tissue samples were collected from the rats for biochemical and histopathological analyses. Antioxidant capacity and proinflammatory cytokine analyses were performed on the blood samples. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed to determine the number of cells undergoing apoptosis in the kidney tissue.
UNASSIGNED: The estimated glomerular filtration rate, an indicator of renal function, was lower in the IR group (p1 = 0.024 vs. p3 = 0.041, respectively) compared to the other groups, while creatinine levels were higher in the IR group compared to the other groups (p1 = 0.032 vs. p2 = 0.044, respectively). The blood urea nitrogen level was higher in the IR group than in the other groups (p1 = 0.001vs p2 = 0.035, respectively). The total antioxidant and total oxidant status, indicating tissue oxidative stress, did not differ between groups (p = 0.914 vs. p = 0.184, respectively). Among the proinflammatory cytokines, the interleukin-1β (IL-1β) and IL-6 levels were significantly higher in the IR group (p = 0.034 vs. p = 0.001, respectively), but the tumour necrosis factor-α (p = 0.19), and interferon-γ (p = 0.311) levels did not differ between groups. Histopathological examination showed significantly less damage to glomerular and tubular cells in the IR-safranal group (p < 0.001). The number of TUNEL-positive cells was higher in the IR group compared to the other groups (p < 0.001).
UNASSIGNED: Safranal may have protective effects against kidney damage caused by distant ischemia-reperfusion injury.
UNASSIGNED: Used in this study were 24 Wistar Albino male rats, which were divided into 3 equal and randomised groups. The sham group underwent laparotomy only. In the IR group, the infrarenal aorta was clamped for 1 h, and then reperfused for 2 h. In the IR-safranal group, safranal was administered 30 min before the procedure and IR injury was induced in the same way as in the IR group. After the procedure, blood and tissue samples were collected from the rats for biochemical and histopathological analyses. Antioxidant capacity and proinflammatory cytokine analyses were performed on the blood samples. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed to determine the number of cells undergoing apoptosis in the kidney tissue.
UNASSIGNED: The estimated glomerular filtration rate, an indicator of renal function, was lower in the IR group (p1 = 0.024 vs. p3 = 0.041, respectively) compared to the other groups, while creatinine levels were higher in the IR group compared to the other groups (p1 = 0.032 vs. p2 = 0.044, respectively). The blood urea nitrogen level was higher in the IR group than in the other groups (p1 = 0.001vs p2 = 0.035, respectively). The total antioxidant and total oxidant status, indicating tissue oxidative stress, did not differ between groups (p = 0.914 vs. p = 0.184, respectively). Among the proinflammatory cytokines, the interleukin-1β (IL-1β) and IL-6 levels were significantly higher in the IR group (p = 0.034 vs. p = 0.001, respectively), but the tumour necrosis factor-α (p = 0.19), and interferon-γ (p = 0.311) levels did not differ between groups. Histopathological examination showed significantly less damage to glomerular and tubular cells in the IR-safranal group (p < 0.001). The number of TUNEL-positive cells was higher in the IR group compared to the other groups (p < 0.001).
UNASSIGNED: Safranal may have protective effects against kidney damage caused by distant ischemia-reperfusion injury.
摘要：
■对身体一部分的缺血再灌注（IR）损伤会对肾脏和心脏等远处器官造成损害。本研究探讨了savranal对IR诱导的肾损伤的保护作用。
■本研究中使用的是24只Wistar白化病雄性大鼠，分为3个相等和随机组。假手术组仅进行剖腹手术。在IR组中,肾下主动脉夹住1小时，然后再灌注2小时。在IR-Safranal组中，手术前30分钟给药safranal，并以与IR组相同的方式诱导IR损伤。手术后,收集大鼠血液和组织样本进行生化和组织病理学分析.对血液样品进行抗氧化能力和促炎细胞因子分析。进行末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TUNEL)染色以确定肾组织中经历凋亡的细胞数量。
■估计的肾小球滤过率，肾功能的指标,在IR组中较低(p1=0.024vs.p3=0.041)与其他组相比，而与其他组相比，IR组的肌酐水平更高(p1=0.032vs.p2分别=0.044)。IR组的血尿素氮水平高于其他组（p1=0.001vsp2=0.035）。总抗氧化剂和总氧化剂状态，指示组织氧化应激，组间没有差异(p=0.914vs.分别为p=0.184)。在促炎细胞因子中，IR组的白细胞介素-1β（IL-1β）和IL-6水平显着升高（p=0.034vs.p=0.001，分别），但肿瘤坏死因子-α（p=0.19），和干扰素-γ（p=0.311）水平在组间没有差异。组织病理学检查显示，在IR-savranal组中，肾小球和肾小管细胞的损伤明显减少（p<0.001）。与其他组相比,IR组中TUNEL阳性细胞的数量更高(p<0.001)。
■Safranal可能对远处缺血再灌注损伤引起的肾脏损伤具有保护作用。
■本研究中使用的是24只Wistar白化病雄性大鼠，分为3个相等和随机组。假手术组仅进行剖腹手术。在IR组中,肾下主动脉夹住1小时，然后再灌注2小时。在IR-Safranal组中，手术前30分钟给药safranal，并以与IR组相同的方式诱导IR损伤。手术后,收集大鼠血液和组织样本进行生化和组织病理学分析.对血液样品进行抗氧化能力和促炎细胞因子分析。进行末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TUNEL)染色以确定肾组织中经历凋亡的细胞数量。
■估计的肾小球滤过率，肾功能的指标,在IR组中较低(p1=0.024vs.p3=0.041)与其他组相比，而与其他组相比，IR组的肌酐水平更高(p1=0.032vs.p2分别=0.044)。IR组的血尿素氮水平高于其他组（p1=0.001vsp2=0.035）。总抗氧化剂和总氧化剂状态，指示组织氧化应激，组间没有差异(p=0.914vs.分别为p=0.184)。在促炎细胞因子中，IR组的白细胞介素-1β（IL-1β）和IL-6水平显着升高（p=0.034vs.p=0.001，分别），但肿瘤坏死因子-α（p=0.19），和干扰素-γ（p=0.311）水平在组间没有差异。组织病理学检查显示，在IR-savranal组中，肾小球和肾小管细胞的损伤明显减少（p<0.001）。与其他组相比,IR组中TUNEL阳性细胞的数量更高(p<0.001)。
■Safranal可能对远处缺血再灌注损伤引起的肾脏损伤具有保护作用。
