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Abstract：
Periodontitis is an infection-driven inflammatory condition of the periodontium. Neutrophils are one of the most important first-line immune cells that protect against pathogen microorganisms in the saliva, but they may also mediate tissue death in inflammatory disorders. The aim of our study was to estimate salivary levels of azurocidin and extracellular azurophilic granules cluster of differentiation (CD63) as biomarkers of neutrophil activation in patients with periodontal diseases and to study the correlation between the levels of these two biomarkers and clinical periodontal parameters. The study included 60 patients with periodontal disease (30 patients with periodontitis and 30 with gingivitis) and 25 healthy controls. The assessed parameters were bleeding on probing, the plaque index, clinical attachment loss, and probing pocket depth. Saliva samples were taken from each study participant, and azurocidin and CD63 levels were measured using ELISA. Azurocidin and CD63 levels were significantly higher in patients with periodontitis and patients with gingivitis than in controls (P < 0.05), and significantly higher in patients with periodontitis than in patients with gingivitis (P < 0.05). Moreover, we found a significant positive correlation between the two biomarkers with clinical attachment loss in the periodontitis group. This study has shown that increased salivary azurocidin and extracellular CD63 levels are associated with enhanced innate response in periodontal disease and can be considered biomarkers of neutrophil activation.
摘要：
牙周炎是一种感染驱动的牙周炎性疾病。中性粒细胞是最重要的一线免疫细胞之一，可以抵抗唾液中的病原体微生物，但它们也可能介导炎症性疾病中的组织死亡。我们研究的目的是评估牙周病患者唾液中作为中性粒细胞激活的生物标志物的天青素和细胞外天青颗粒分化簇（CD63）的水平，并研究这两种生物标志物的水平与临床牙周参数之间的相关性。该研究包括60名牙周病患者（30名牙周炎患者和30名牙龈炎患者）和25名健康对照。评估的参数是探查时出血，菌斑指数,临床附着丧失，和探测口袋深度。唾液样本取自每个研究参与者，使用ELISA测量天青素和CD63水平。牙周炎患者和牙龈炎患者的Azurocidin和CD63水平明显高于对照组（P<0.05），牙周炎患者明显高于牙龈炎患者(P<0.05)。此外，我们发现两种生物标志物与牙周炎组临床附着丧失之间存在显著正相关.这项研究表明，唾液天青素和细胞外CD63水平的增加与牙周病的先天反应增强有关，可以被认为是中性粒细胞活化的生物标志物。
