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Abstract：
Numerous studies have established a link between gene variants within the inflammasome complex and the incidence of periodontitis and cardiovascular illness across various ethnic groups. This study investigated the association between PYCARD gene polymorphism and susceptibility to periodontal disease and coronary heart disease (CHD) and their correlation with clinical periodontal indices. A total of 120 participants were enrolled, categorized into four groups: 30 healthy controls (C), 30 patients with generalized periodontitis (P), 30 patients with atherosclerotic CHD but clinically healthy periodontium (AS-C), and 30 patients with both atherosclerotic CHD and generalized periodontitis (AS-P). We recorded demographic data, collected blood samples, and measured periodontal indices, including plaque index, clinical attachment loss, bleeding on probing, and pocket depth. The genomic variant of the PYCARD gene was analyzed using a conventional polymerase reaction. A significant prevalence of T and G allele mutations and a higher distribution of CT and TT genotypes in PYCARD C/T (rs8056505) and the AG genotype in PYCARD A/G (rs372507365) were observed in groups P, AS-P, and AS-C. These single nucleotide polymorphisms (SNPs) were also positively correlated with the severity of clinical periodontitis indices. Our findings suggest that the increased frequency of T and G alleles and the distribution of CT, TT, and AG genotypes in PYCARD SNPs are significantly associated with an elevated risk for periodontal disease and CHD. These SNPs may participate in the pathogenesis of these conditions. The study reinforces the potential role of these genetic markers as risk factors for both diseases in the Iraqi population.
摘要：
许多研究已经建立了炎症复合体内的基因变异与不同种族的牙周炎和心血管疾病发病率之间的联系。本研究探讨PYCARD基因多态性与牙周病和冠心病(CHD)易感性的关系及其与临床牙周指标的相关性。共有120名参与者报名参加，分为四组：30名健康对照（C），30例广泛性牙周炎（P），30例动脉粥样硬化性冠心病，但临床健康的牙周组织（AS-C），和30名同时患有动脉粥样硬化性CHD和广泛性牙周炎（AS-P）的患者。我们记录了人口统计数据,采集血液样本,测量牙周指数，包括菌斑指数,临床附着丧失，探查时出血,和口袋深度。使用常规聚合酶反应分析PYCARD基因的基因组变体。在P组中观察到T和G等位基因突变的显着患病率以及PYCARDC/T（rs8056505）和PYCARDA/G（rs372507365）中CT和TT基因型的分布较高，AS-P,和AS-C这些单核苷酸多态性（SNP）也与临床牙周炎指数的严重程度呈正相关。我们的研究结果表明，T和G等位基因的频率增加和CT的分布，TT,PYCARDSNP中的AG基因型与牙周病和CHD风险升高显著相关。这些SNP可能参与这些病症的发病机理。该研究加强了这些遗传标记作为伊拉克人口中两种疾病的危险因素的潜在作用。
