PDF(Pubmed)
Abstract：
UNASSIGNED: Chemical biological radiological nuclear threats are at an important point in the agenda of world health today, as they can cause mass deaths. B. mallei attracts attention as a potential biological warfare agent due to its features such as multidrug resistance, a rapid transmission mechanism via aerosol, the absence of a complete treatment protocol for the infection it causes, and the absence of an approved vaccine for protection against the bacteria. B. mallei suspect samples must be studied by experienced personnel in biosafety level III laboratories. B mallei is a difficult and troublesome pathogen to diagnose and many unknowns about B. mallei today. Therefore, the aim of the study was to determine the molecular differences and potential resistance genes of B mallei strains.
UNASSIGNED: Determination of the molecular differences and potential resistance genes of B mallei strains with new bioinformatics approaches by comparatively examining the data of 29 B mallei strains, 10 of which were isolated from Türkiye, on the genome list of the National Biotechnology Information Center (NCBI).
UNASSIGNED: According to the genome annotations of the origins, the origin containing the highest number of CDS which is 5172 was found as the 11th strain obtained in Türkiye in 1949. The origin with the highest number of pseudogenes was determined as 23,344 (China 7) origin. Two hundred and eighty-five pseudogenes found in this strain were obtained from a knee effusion in Myanmar. According to chromosome 2 data, B. mallei strain was determined as the most similar strain to ATCC 23344, line 11 with NCTC 10229 strain, and SAVP1 strain was determined as the least similar strain. When the antimicrobial resistance gene markers of the isolates included in the study were examined, amrA and amrB, qacG ade, Burkholderia pseudomallei Omp38 were found to be carrying.
UNASSIGNED: In terms of public health, it was thought that the data obtained as a result of our study about B mallei, which is defined as a biological weapon, is very valuable for creating treatment protocols to be applied to possible epidemics in the future. In addition, the available genetic epidemiological data of these strains belonging to a category that is dangerous to work with in a laboratory environment were reviewed.
UNASSIGNED: Determination of the molecular differences and potential resistance genes of B mallei strains with new bioinformatics approaches by comparatively examining the data of 29 B mallei strains, 10 of which were isolated from Türkiye, on the genome list of the National Biotechnology Information Center (NCBI).
UNASSIGNED: According to the genome annotations of the origins, the origin containing the highest number of CDS which is 5172 was found as the 11th strain obtained in Türkiye in 1949. The origin with the highest number of pseudogenes was determined as 23,344 (China 7) origin. Two hundred and eighty-five pseudogenes found in this strain were obtained from a knee effusion in Myanmar. According to chromosome 2 data, B. mallei strain was determined as the most similar strain to ATCC 23344, line 11 with NCTC 10229 strain, and SAVP1 strain was determined as the least similar strain. When the antimicrobial resistance gene markers of the isolates included in the study were examined, amrA and amrB, qacG ade, Burkholderia pseudomallei Omp38 were found to be carrying.
UNASSIGNED: In terms of public health, it was thought that the data obtained as a result of our study about B mallei, which is defined as a biological weapon, is very valuable for creating treatment protocols to be applied to possible epidemics in the future. In addition, the available genetic epidemiological data of these strains belonging to a category that is dangerous to work with in a laboratory environment were reviewed.
摘要：
■化学生物放射性核威胁是当今世界卫生议程中的重要时刻，因为它们可能导致大规模死亡。B.mallei作为一种潜在的生物战剂，由于其多药耐药性等特征而引起了人们的注意，通过气溶胶的快速传播机制，缺乏针对其引起的感染的完整治疗方案，以及缺乏经批准的针对细菌的疫苗。B.mallei可疑样品必须由生物安全III级实验室的经验丰富的人员进行研究。B.mallei是一种难以诊断且麻烦的病原体，并且当今关于B.mallei的许多未知知识。因此,该研究的目的是确定Bmallei菌株的分子差异和潜在的抗性基因。
■用新的生物信息学方法，通过比较29个Bmallei菌株的数据，确定Bmallei菌株的分子差异和潜在的抗性基因，其中10个是从蒂尔基耶分离出来的，在国家生物技术信息中心（NCBI）的基因组列表上。
■根据起源的基因组注释，1949年在Türkiye获得的第11种菌株发现了含有最高数量的CDS的来源，即5172。假基因数量最多的起源被确定为23,344（中国7）起源。在该菌株中发现的二百八十五个假基因是从缅甸的膝盖积液中获得的。根据2号染色体的数据,B.mallei菌株被确定为与ATCC23344，第11行NCTC10229菌株最相似的菌株，和SAVP1菌株被确定为最不相似的菌株。当检查研究中包括的分离株的抗菌素抗性基因标记时，amrA和amrB,qacGade,发现假伯克霍尔德氏菌Omp38携带。
■在公共卫生方面，据认为，作为我们研究的结果获得的数据，被定义为生物武器，对于创建将来应用于可能的流行病的治疗方案非常有价值。此外,我们审查了这些菌株的遗传流行病学数据，这些菌株属于在实验室环境中工作危险的类别。
■用新的生物信息学方法，通过比较29个Bmallei菌株的数据，确定Bmallei菌株的分子差异和潜在的抗性基因，其中10个是从蒂尔基耶分离出来的，在国家生物技术信息中心（NCBI）的基因组列表上。
■根据起源的基因组注释，1949年在Türkiye获得的第11种菌株发现了含有最高数量的CDS的来源，即5172。假基因数量最多的起源被确定为23,344（中国7）起源。在该菌株中发现的二百八十五个假基因是从缅甸的膝盖积液中获得的。根据2号染色体的数据,B.mallei菌株被确定为与ATCC23344，第11行NCTC10229菌株最相似的菌株，和SAVP1菌株被确定为最不相似的菌株。当检查研究中包括的分离株的抗菌素抗性基因标记时，amrA和amrB,qacGade,发现假伯克霍尔德氏菌Omp38携带。
■在公共卫生方面，据认为，作为我们研究的结果获得的数据，被定义为生物武器，对于创建将来应用于可能的流行病的治疗方案非常有价值。此外,我们审查了这些菌株的遗传流行病学数据，这些菌株属于在实验室环境中工作危险的类别。
