Abstract：
Pregnancy toxaemia is a serious disease that occurs during the last trimester of pregnancy in sheep. Yet, in most cases, the disease may have a subclinical course. This study was aimed at comparing blood βHBA measurement devices for diagnosis of subclinical pregnancy toxaemia in late pregnant sheep. In the study, a total of 50 blood samples were collected from Romanov (n = 30) and cross-bred Hamdani (n = 20) sheep. Blood βHBA levels were measured using TaiDoc TD-4235 and CentriVet βHBA hand-held meter. Randox βHBA (Ranbut) assay was used as a reference laboratory method to compare hand-held meters. βHBA value of 0.8 mmol/L was set as the cut-off value for diagnosis of subclinical pregnancy toxaemia. Statistical analyses were carried out using Minitab 21 and Jamovi software. In the study, the correlation of Randox-TaiDoc TD-4235 and Randox-CentriVet was .822 (p < .001) and .728 (p < .001), respectively. Based on the Ranbut assay, nine ewes out of 50 were diagnosed with subclinical pregnancy toxaemia. Specificity (detection of healthy ewes) and sensitivity (detection of ewes with subclinical pregnancy toxaemia) for TaiDoc TD-4235 and CentriVet hand-held meters were 100%, 77.8%, and 100%, 66.7%, respectively. In the receiver operating characteristic (ROC) analysis, areas under the ROC curve (AUC) were 0.976 and 0.920 for TaiDoc and CentriVet, respectively. Bland-Altman analysis revealed a bias of 0.092 mmol/L for TaiDoc and a bias of 0.132 mmol/L for CentriVet. TaiDoc hand-held meter shows a better correlation with the Randox Ranbut assay and greater sensitivity compared to the CentriVet hand-held meter. In conclusion, both TaiDoc and CentriVet hand-held meters can be securely used in the diagnosis of subclinical pregnancy toxaemia in sheep. For these reasons, subclinical pregnancy toxaemia and these devices will be evaluated within the scope of herd management programme in the sheep industry. It should also be taken into account that these conditions will affect the future fertility of the mother and offspring.
摘要：
妊娠毒血症是一种严重的疾病，发生在绵羊妊娠的最后三个月。然而，在大多数情况下,该疾病可能有亚临床病程。这项研究旨在比较血液βHBA测量设备，以诊断妊娠晚期绵羊的亚临床妊娠毒血症。在研究中,从Romanov(n=30)和杂交的Hamdani(n=20)绵羊中收集了50份血样.使用TaiDocTD-4235和CentriVetβHBA手持式测量仪测量血液βHBA水平。RandoxβHBA（Ranbut）测定法用作参考实验室方法，以比较手持式仪表。βHBA值0.8mmol/L作为诊断亚临床妊娠毒血症的临界值。使用Minitab21和Jamovi软件进行统计分析。在研究中,Randox-TaiDocTD-4235和Randox-CentriVet的相关性为.822(p<.001)和.728(p<.001)，分别。根据Ranbut试验,50只母羊中有9只被诊断患有亚临床妊娠毒血症。TaiDocTD-4235和CentriVet手持仪的特异性（健康母羊的检测）和敏感性（亚临床妊娠毒血症母羊的检测）均为100％，77.8%,100%,66.7%,分别。在接收机工作特性(ROC)分析中，TaiDoc和CentriVet的ROC曲线下面积（AUC）分别为0.976和0.920，分别。Bland-Altman分析显示，TaiDoc的偏差为0.092mmol/L，CentriVet的偏差为0.132mmol/L。与CentriVet手持式仪表相比，TaiDoc手持式仪表与RandoxRanbut测定具有更好的相关性，并且灵敏度更高。总之,TaiDoc和CentriVet手持式仪表均可安全地用于诊断绵羊的亚临床妊娠毒血症。由于这些原因,亚临床妊娠毒血症和这些装置将在羊群管理计划的范围内进行评估。还应考虑到这些条件将影响母亲和后代的未来生育能力。
