Abstract：
BACKGROUND: Melanogenesis, regulated by genetic, hormonal, and environmental factors, occurs in melanocytes in the basal layer of the epidermis. Dysregulation of this process can lead to various skin disorders, such as hyperpigmentation and hypopigmentation. Therefore, the present study investigated the effect of ultrasonic-assisted ethanol extract (SHUE) from Sargassum horneri (S. horneri), brown seaweed against melanogenesis in α-melanocyte-stimulating hormone (MSH)-stimulated B16F10 murine melanocytes.
METHODS: Firstly, yield and proximate compositional analysis of the samples were conducted. The effect of SHUE on cell viability has been evaluated by using 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. After that, the melanin content and cellular tyrosinase activity in α-MSH-stimulated B16F10 murine melanocytes were examined. Western blot analysis was carried out to investigate the protein expression levels of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP1), and tyrosinase-related protein-2 (TRP2). In addition, the effect of extracellular signal-regulated kinase (ERK) on the melanogenesis process was assessed via Western blotting.
RESULTS: As per the analysis, SHUE contained the highest average yield on a dry basis at 28.70 ± 3.21%. The findings showed that SHUE reduced the melanin content and cellular tyrosinase activity in α-MSH-stimulated B16F10 murine melanocytes. Additionally, the expression levels of MITF, TRP1, and TRP2 protein were significantly downregulated by SHUE treatment in α-MSH-stimulated B16F10 murine melanocytes. Moreover, SHUE upregulated the phosphorylation of ERK and AKT in α-MSH-stimulated B16F10 murine melanocytes. In addition, experiments conducted using the ERK inhibitor (PD98059) revealed that the activity of SHUE depends on the ERK signaling cascade.
CONCLUSIONS: These results suggest that SHUE has an anti-melanogenic effect and can be used as a material in the formulation of cosmetics related to whitening and lightening.
METHODS: Firstly, yield and proximate compositional analysis of the samples were conducted. The effect of SHUE on cell viability has been evaluated by using 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. After that, the melanin content and cellular tyrosinase activity in α-MSH-stimulated B16F10 murine melanocytes were examined. Western blot analysis was carried out to investigate the protein expression levels of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP1), and tyrosinase-related protein-2 (TRP2). In addition, the effect of extracellular signal-regulated kinase (ERK) on the melanogenesis process was assessed via Western blotting.
RESULTS: As per the analysis, SHUE contained the highest average yield on a dry basis at 28.70 ± 3.21%. The findings showed that SHUE reduced the melanin content and cellular tyrosinase activity in α-MSH-stimulated B16F10 murine melanocytes. Additionally, the expression levels of MITF, TRP1, and TRP2 protein were significantly downregulated by SHUE treatment in α-MSH-stimulated B16F10 murine melanocytes. Moreover, SHUE upregulated the phosphorylation of ERK and AKT in α-MSH-stimulated B16F10 murine melanocytes. In addition, experiments conducted using the ERK inhibitor (PD98059) revealed that the activity of SHUE depends on the ERK signaling cascade.
CONCLUSIONS: These results suggest that SHUE has an anti-melanogenic effect and can be used as a material in the formulation of cosmetics related to whitening and lightening.
摘要：
背景：黑色素发生，受基因调控，荷尔蒙，和环境因素，发生在表皮基底层的黑素细胞中。这个过程的失调会导致各种皮肤病，如色素沉着过度和色素沉着不足。因此,本研究调查了马尾藻超声波辅助乙醇提取物(SHUE)的作用(S.horneri),褐藻在α-黑素细胞刺激激素（MSH）刺激的B16F10鼠黑素细胞中对抗黑素生成。
方法：首先，进行了样品的产量和近似组成分析。SHUE对细胞活力的影响已通过使用3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-四唑溴化物（MTT）测定法进行了评估。之后,检测了α-MSH刺激的B16F10鼠黑素细胞中的黑色素含量和细胞酪氨酸酶活性。Westernblot分析小眼症相关转录因子(MITF)的蛋白表达水平,酪氨酸酶,酪氨酸酶相关蛋白-1（TRP1），和酪氨酸酶相关蛋白-2(TRP2)。此外,细胞外信号调节激酶(ERK)对黑素生成过程的影响通过Western印迹进行评估.
结果：根据分析，SHUE的干基平均产量最高，为28.70±3.21％。结果表明，SHUE降低了α-MSH刺激的B16F10鼠黑素细胞中的黑色素含量和细胞酪氨酸酶活性。此外,MITF的表达水平，在α-MSH刺激的B16F10鼠黑素细胞中，通过SHUE处理，TRP1和TRP2蛋白显着下调。此外，SHUE上调α-MSH刺激的B16F10鼠黑素细胞中ERK和AKT的磷酸化。此外,使用ERK抑制剂（PD98059）进行的实验表明，SHUE的活性取决于ERK信号级联。
结论：这些结果表明SHUE具有抗黑色素生成作用，可用作与美白和美白有关的化妆品配方中的材料。
方法：首先，进行了样品的产量和近似组成分析。SHUE对细胞活力的影响已通过使用3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-四唑溴化物（MTT）测定法进行了评估。之后,检测了α-MSH刺激的B16F10鼠黑素细胞中的黑色素含量和细胞酪氨酸酶活性。Westernblot分析小眼症相关转录因子(MITF)的蛋白表达水平,酪氨酸酶,酪氨酸酶相关蛋白-1（TRP1），和酪氨酸酶相关蛋白-2(TRP2)。此外,细胞外信号调节激酶(ERK)对黑素生成过程的影响通过Western印迹进行评估.
结果：根据分析，SHUE的干基平均产量最高，为28.70±3.21％。结果表明，SHUE降低了α-MSH刺激的B16F10鼠黑素细胞中的黑色素含量和细胞酪氨酸酶活性。此外,MITF的表达水平，在α-MSH刺激的B16F10鼠黑素细胞中，通过SHUE处理，TRP1和TRP2蛋白显着下调。此外，SHUE上调α-MSH刺激的B16F10鼠黑素细胞中ERK和AKT的磷酸化。此外,使用ERK抑制剂（PD98059）进行的实验表明，SHUE的活性取决于ERK信号级联。
结论：这些结果表明SHUE具有抗黑色素生成作用，可用作与美白和美白有关的化妆品配方中的材料。
