Abstract：
Rhei Radix et Rhizoma is common traditional Chinese medicine with multiple original plants. The content and proportion of the active components in Rhei Radix et Rhizoma from different plant species were compared to accurately evaluate the medicine qua-lity and provide a theoretical basis for precise use of this medicine in clinical practice. In this study, fresh Rhei Radix et Rhizoma samples were collected from the four-year-old plants of Rheum palmatum, R. tanguticum, and R. officinale. The relative content of 220 anthraquinones, anthrones, and tannins in the samples were determined by pseudo-targeted metabolomics, and the differential components were screened by multivariate statistical methods. The principal component analysis classified the samples into three clusters according to the original plants. The orthogonal partial least squares-discriminant analysis(OPLS-DA) screened out 117 differential components, including 8 free anthraquinones, 18 anthraquinone glycosides, 80 anthrones, and 11 tannins. Twenty-eight components had the highest content in R. tanguticum, mainly including sennosides, anthraquinone glycosides, and procyanidins. Thirty-five components showed the highest content in R. officinale, mainly including free anthraquinones and catechines. Fifty-four components showed the highest content in R. palmatum, mainly including dianthrones, while the structures of most of them cannot be determined temporarily. The content distribution of differential components in the three original plants indicates that R. tanguticum has the strongest effect of purging, while R. officinale has the strongest effect of clearing heat and purging fire, and both have stronger effects of resolvong stasis and dredging meridians than R. palmatum.
摘要：
大黄是常见的中药，具有多种原植物。通过比较不同植物品种大黄中活性成分的含量和比例，准确评价其药物质量，为临床准确使用该药提供理论依据。在这项研究中,从掌叶大黄的四岁植物中收集新鲜的大黄样品，R.tangutum,和R.officinale.蒽醌的相对含量220，anthrones,通过假靶向代谢组学测定样品中的单宁，用多元统计方法筛选差异成分。主成分分析根据原始植物将样品分为三个簇。正交偏最小二乘判别分析(OPLS-DA)筛选出117个差分分量,包括8个游离蒽醌,18蒽醌苷，80个anthrones,还有11种单宁.二十八种成分中含量最高。主要包括森诺斯，蒽醌苷，和原花青素.35种成分含量最高。主要包括游离蒽醌和儿茶素。54种成分在掌叶芦苇中含量最高，主要包括蒽醌,而其中大多数的结构暂时无法确定。三种原始植物中差异成分的含量分布表明，唐古汀的净化效果最强，而R.officinale具有最强的清热和清除火灾的效果，两者的解郁和疏通经络作用均强于掌叶草。
