PDF(Pubmed)
Abstract：
BACKGROUND: This study explores the biosynthesis, characteristics, and functional properties of exopolysaccharide produced by the strain Liquorilactobacillus mali T6-52. The strain demonstrated significant EPS production with a non-ropy phenotype.
RESULTS: The genomic analysis unveiled genes associated with EPS biosynthesis, shedding light on the mechanism behind EPS production. These genes suggest a robust EPS production mechanism, providing insights into the strain\'s adaptability and ecological niche. Chemical composition analysis identified the EPS as a homopolysaccharide primarily composed of glucose, confirming its dextran nature. Furthermore, it demonstrated notable functional properties, including antioxidant activity, fat absorption capacity, and emulsifying activity. Moreover, the EPS displayed promising cryoprotective activities, showing notable performance comparable to standard cryoprotective agents. The EPS concentration also demonstrated significant freeze-drying protective effects, presenting it as a potential alternative cryoprotectant for bacterial storage.
CONCLUSIONS: The functional properties of L. mali T6-52 EPS reveal promising opportunities across various industrial domains. The strain\'s safety profile, antioxidant prowess, and exceptional cryoprotective and freeze-drying characteristics position it as an asset in food processing and pharmaceuticals.
RESULTS: The genomic analysis unveiled genes associated with EPS biosynthesis, shedding light on the mechanism behind EPS production. These genes suggest a robust EPS production mechanism, providing insights into the strain\'s adaptability and ecological niche. Chemical composition analysis identified the EPS as a homopolysaccharide primarily composed of glucose, confirming its dextran nature. Furthermore, it demonstrated notable functional properties, including antioxidant activity, fat absorption capacity, and emulsifying activity. Moreover, the EPS displayed promising cryoprotective activities, showing notable performance comparable to standard cryoprotective agents. The EPS concentration also demonstrated significant freeze-drying protective effects, presenting it as a potential alternative cryoprotectant for bacterial storage.
CONCLUSIONS: The functional properties of L. mali T6-52 EPS reveal promising opportunities across various industrial domains. The strain\'s safety profile, antioxidant prowess, and exceptional cryoprotective and freeze-drying characteristics position it as an asset in food processing and pharmaceuticals.
摘要：
背景：这项研究探索了生物合成，特点,和由马氏甘草芽孢杆菌T6-52菌株产生的胞外多糖的功能特性。该菌株表现出显著的EPS产生,具有非各向异性表型。
结果：基因组分析揭示了与EPS生物合成相关的基因，阐明EPS生产背后的机制。这些基因表明了一种强大的EPS产生机制，提供对菌株适应性和生态位的见解。化学成分分析确定EPS为主要由葡萄糖组成的均多糖，证实了它的葡聚糖性质.此外，它表现出显著的功能特性，包括抗氧化活性，脂肪吸收能力，和乳化活性。此外，EPS显示出有希望的冷冻保护活性，表现出与标准冷冻保护剂相当的显著性能。EPS浓度也表现出显著的冷冻干燥保护作用，将其作为细菌储存的潜在替代冷冻保护剂。
结论：MaliT6-52EPS的功能特性揭示了各种工业领域的有希望的机会。菌株的安全概况，抗氧化能力，以及出色的冷冻保护和冷冻干燥特性使其成为食品加工和制药领域的资产。
结果：基因组分析揭示了与EPS生物合成相关的基因，阐明EPS生产背后的机制。这些基因表明了一种强大的EPS产生机制，提供对菌株适应性和生态位的见解。化学成分分析确定EPS为主要由葡萄糖组成的均多糖，证实了它的葡聚糖性质.此外，它表现出显著的功能特性，包括抗氧化活性，脂肪吸收能力，和乳化活性。此外，EPS显示出有希望的冷冻保护活性，表现出与标准冷冻保护剂相当的显著性能。EPS浓度也表现出显著的冷冻干燥保护作用，将其作为细菌储存的潜在替代冷冻保护剂。
结论：MaliT6-52EPS的功能特性揭示了各种工业领域的有希望的机会。菌株的安全概况，抗氧化能力，以及出色的冷冻保护和冷冻干燥特性使其成为食品加工和制药领域的资产。
