Abstract：
Alveolar echinococcosis is considered to be one of the most potentially lethal parasitic zoonotic diseases. However, the molecular mechanisms by which Echinococcus multilocularis interacts with hosts are poorly understood, hindering the prevention and treatment of this disease. Due to the great advantages of cell culture systems for molecular research, numerous attempts have been made to establish primary cell cultures for E. multilocularis. In this study we developed a simple, rapid, and economical method that allows E. multilocularis metacestode tissue blocks to generate daughter vesicles without the continuous presence of host feeder cells in a regular medium. We performed anaerobic, hypoxic (1% O2), normoxic, and semi-anaerobic (in sealed tubes) cultures and found that E. multilocularis metacestode tissues can produce daughter vesicles only in the sealed tubes after 4 wk of incubation. The daughter vesicles cultivated in this system were remarkably enlarged under anaerobic conditions after 8 days of culture, whereas vesicles cultured under hypoxic (1% O2) and normoxic conditions showed only a mild increase in volume. Our in vitro cultivated vesicles showed strong viability and could be used to test antiparasitic drugs, isolate primary cells, and infect animals.
摘要：
肺泡棘球蚴病被认为是最可能致命的寄生虫人畜共患病之一。然而,多房棘球蚴与宿主相互作用的分子机制知之甚少，阻碍了这种疾病的预防和治疗。由于细胞培养系统用于分子研究的巨大优势，已经进行了许多尝试来建立多房性大肠杆菌的原代细胞培养物。在这项研究中，我们开发了一个简单的，快速,和经济的方法，该方法允许多房性E.我们做了厌氧,低氧(1%O2),常氧,和半厌氧（在密封管中）培养，并发现多房性E.在该系统中培养的子囊泡在厌氧条件下培养8天后明显扩大，而在低氧（1％O2）和常氧条件下培养的囊泡仅显示体积的轻度增加。我们在体外培养的囊泡显示出很强的生存能力，可用于测试抗寄生虫药物，分离原代细胞，感染动物。
