Abstract：
Plants of the Garcinia genus were rich in structurally diverse and naturally bioactive components, while limited studies have been reported for Garcinia pedunculata Roxb. and G. nujiangensis C. Y. Wu & Y. H. Li. Four previously undescribed compounds including three chromones, garpedunchromones A-C (1-3), and one biflavonoid, nujiangbiflavone A (14), along with fifteen known analogs (4-13, 15-19) were isolated from G. pedunculata and G. nujiangensis. The structures of the isolated compounds were determined based on their HRESIMS data, extensive NMR spectroscopic analyses, and ECD calculations. The chromone derivatives were isolated from Garcinia for the first time. Compound 14 was a rare biflavonoid with C-3─C-6″ linkage. The biological evaluation of these isolates against NO production was conducted in the LPS-induced RAW 264.7 cells, resulting in the identification of a series of potent NO inhibitors, of which garpedunchromone B (2) was the most active with an IC50 value of 18.11 ± 0.96 μM. In the network pharmacology studies, the potential targets of compounds and inflammation were obtained from PharmMapper and GeneCards database. GO and KEGG enrichment analysis revealed that the overlapped targets were closely related to the major pathogenic processes linked to inflammation. Garpedunchromone B and proteins binding sites were being predicted.
摘要：
藤黄属植物富含结构多样和天然生物活性成分，虽然对藤黄的研究有限。吴和李。四种先前未描述的化合物，包括三种色酮，garpedunchemesA-C（1-3），和一种双类黄酮，努江黄酮A（14），与15种已知的类似物（4-13，15-19）一起，从花梗G.根据HRESIMS数据确定分离化合物的结构，广泛的核磁共振光谱分析，和ECD计算。色酮衍生物为首次从藤黄中分离得到。化合物14是一种罕见的双类黄酮，具有C-3─C-6″键。在LPS诱导的RAW264.7细胞中进行了这些分离株对NO产生的生物学评估，从而鉴定出一系列有效的NO抑制剂，其中gargedunchromoneB（2）最活跃，IC50值为18.11±0.96μM。在网络药理学研究中，化合物和炎症的潜在靶标来自PharmMapper和GeneCards数据库.GO和KEGG富集分析显示，重叠的靶标与炎症相关的主要致病过程密切相关。GripedunromoneB和蛋白质结合位点正在被预测。
