Abstract：
BACKGROUND: Peripheral blood stem cells (PBSC) mobilization with granulocyte colony stimulating factor (G-CSF) for healthy donors is generally performed at 5th day. However, earlier collection is sometimes feasible, raising the question of whether to initiate apheresis early to limit further G-CSF exposure, while considering the risk of mobilization failure. In the current study, we examined the factors predicting successful 4th day collection and developed a model that can be used practically.
METHODS: The study was carried out by obtaining the data of PBSC mobilizations performed between January 2009 and September 2022 in our transplantation center.
RESULTS: A total of 141 healthy donors with a median donor age of 32 (18-64) were included. Adequate mobilization was achieved in 115 (81.6 %) patients. Median peripheral CD34 + cell count was 69.4/μL in the adequate mobilization group and 46/μL in the mobilization failure group (p < 0001). Multivariate analysis revealed that donor/recipient weight ratio and the 4th day peripheral CD34 + cell count≥ 50/μL were independent markers for 4th day collection success. A predictive model of our center including these parameters was available with 0.765 sensitivity and 0.968 specificity [(AUC):0.948 (95 % CI, 0.90-0.99), p < 0.001].
CONCLUSIONS: The result of the current study shows that peripheral 4th day collection can be performed in selected donors, taking into account peripheral CD34+ cell count and donor/recipient weight ratio. In addition, using these indicators, new predictive models can be created that may assist clinicians in daily practice.
METHODS: The study was carried out by obtaining the data of PBSC mobilizations performed between January 2009 and September 2022 in our transplantation center.
RESULTS: A total of 141 healthy donors with a median donor age of 32 (18-64) were included. Adequate mobilization was achieved in 115 (81.6 %) patients. Median peripheral CD34 + cell count was 69.4/μL in the adequate mobilization group and 46/μL in the mobilization failure group (p < 0001). Multivariate analysis revealed that donor/recipient weight ratio and the 4th day peripheral CD34 + cell count≥ 50/μL were independent markers for 4th day collection success. A predictive model of our center including these parameters was available with 0.765 sensitivity and 0.968 specificity [(AUC):0.948 (95 % CI, 0.90-0.99), p < 0.001].
CONCLUSIONS: The result of the current study shows that peripheral 4th day collection can be performed in selected donors, taking into account peripheral CD34+ cell count and donor/recipient weight ratio. In addition, using these indicators, new predictive models can be created that may assist clinicians in daily practice.
摘要：
背景：通常在第5天使用粒细胞集落刺激因子（G-CSF）动员健康供体的外周血干细胞（PBSC）。然而,早期收集有时是可行的，提出了一个问题，即是否尽早开始单采术以限制进一步的G-CSF暴露，同时考虑动员失败的风险。在目前的研究中,我们研究了预测第4天收集成功的因素，并开发了一个可以实际使用的模型。
方法：该研究是通过获取2009年1月至2022年9月在我们的移植中心进行的PBSC动员的数据进行的。
结果：共纳入141名健康供体，供体年龄中位数为32岁（18-64岁）。115例（81.6％）患者实现了足够的动员。在充分动员组中,外周CD34+细胞计数中位数为69.4/μL,在动员失败组中为46/μL(p<0001)。多变量分析显示，供体/受体体重比和第4天外周血CD34细胞计数≥50/μL是第4天收集成功的独立标志物。我们中心的预测模型包括这些参数，具有0.765的灵敏度和0.968的特异性[（AUC）：0.948（95％CI，0.90-0.99），p<0.001]。
结论：当前研究的结果表明，可以在选定的供体中进行第4天的外周采集，考虑到外周CD34+细胞计数和供体/受体体重比。此外,使用这些指标，可以创建新的预测模型，以帮助临床医生进行日常实践。
方法：该研究是通过获取2009年1月至2022年9月在我们的移植中心进行的PBSC动员的数据进行的。
结果：共纳入141名健康供体，供体年龄中位数为32岁（18-64岁）。115例（81.6％）患者实现了足够的动员。在充分动员组中,外周CD34+细胞计数中位数为69.4/μL,在动员失败组中为46/μL(p<0001)。多变量分析显示，供体/受体体重比和第4天外周血CD34细胞计数≥50/μL是第4天收集成功的独立标志物。我们中心的预测模型包括这些参数，具有0.765的灵敏度和0.968的特异性[（AUC）：0.948（95％CI，0.90-0.99），p<0.001]。
结论：当前研究的结果表明，可以在选定的供体中进行第4天的外周采集，考虑到外周CD34+细胞计数和供体/受体体重比。此外,使用这些指标，可以创建新的预测模型，以帮助临床医生进行日常实践。
