PDF(Pubmed)
Abstract：
Biofilms make it difficult to eradicate bacterial infections through antibiotic treatments and lead to numerous complications. Previously, two periprosthetic infection-related pathogens, Enterococcus faecalis and Staphylococcus lugdunensis were reported to have relatively contrasting biofilm-forming abilities. In this study, we examined the proteomics of the two microorganisms\' biofilms using LC-MS/MS. The results showed that each microbe exhibited an overall different profile for differential gene expressions between biofilm and planktonic cells as well as between each other. Of a total of 929 proteins identified in the biofilms of E. faecalis, 870 proteins were shared in biofilm and planktonic cells, and 59 proteins were found only in the biofilm. In S. lugdunensis, a total of 1125 proteins were identified, of which 1072 proteins were found in common in the biofilm and planktonic cells, and 53 proteins were present only in the biofilms. The functional analysis for the proteins identified only in the biofilms using UniProt keywords demonstrated that they were mostly assigned to membrane, transmembrane, and transmembrane helix in both microorganisms, while hydrolase and transferase were found only in E. faecalis. Protein-protein interaction analysis using STRING-db indicated that the resulting networks did not have significantly more interactions than expected. GO term analysis exhibited that the highest number of proteins were assigned to cellular process, catalytic activity, and cellular anatomical entity. KEGG pathway analysis revealed that microbial metabolism in diverse environments was notable for both microorganisms. Taken together, proteomics data discovered in this study present a unique set of biofilm-embedded proteins of each microorganism, providing useful information for diagnostic purposes and the establishment of appropriately tailored treatment strategies. Furthermore, this study has significance in discovering the target candidate molecules to control the biofilm-associated infections of E. faecalis and S. lugdunensis.
摘要：
生物膜使得难以通过抗生素治疗根除细菌感染并导致许多并发症。以前,两种假体周围感染相关病原体，据报道，粪肠球菌和罗氏葡萄球菌具有相对不同的生物膜形成能力。在这项研究中,我们使用LC-MS/MS检查了两种微生物生物膜的蛋白质组学。结果表明，每种微生物在生物膜和浮游细胞之间以及彼此之间表现出总体不同的差异基因表达谱。在粪肠球菌生物膜中鉴定出的929种蛋白质中，870种蛋白质在生物膜和浮游细胞中共享，仅在生物膜中发现了59种蛋白质。在S.Lugdunensis，总共鉴定了1125种蛋白质，其中在生物膜和浮游细胞中发现了1072种蛋白质，53种蛋白质仅存在于生物膜中。使用UniProt关键字对仅在生物膜中鉴定的蛋白质进行的功能分析表明，它们主要被分配给膜，跨膜，以及两种微生物中的跨膜螺旋，而水解酶和转移酶仅在粪肠球菌中发现。使用STRING-db的蛋白质-蛋白质相互作用分析表明，所得网络没有比预期的明显更多的相互作用。GO术语分析显示，最高数量的蛋白质被分配给细胞过程，催化活性，和细胞解剖实体。KEGG途径分析显示，两种微生物在不同环境中的代谢都很明显。一起来看,在这项研究中发现的蛋白质组学数据显示了每种微生物的一组独特的生物膜嵌入蛋白，为诊断目的提供有用的信息，并建立适当的治疗策略。此外，本研究对发现控制粪肠球菌和陆生S.lugdunensis生物膜相关感染的靶候选分子具有重要意义。
