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Abstract：
BACKGROUND: Diagnosis of the majority of hepatocellular carcinoma (HCC) patients occurs at intermediate to advanced stages, with a few curative therapeutic options being available. It is therefore strongly urgent to discover additional adjuvant therapy for this lethal malignancy. This study aimed to assess the effectiveness of curcumin (C), piperine (P) and taurine (T) combination as adjuvant agents on serum levels of IFN-γ, immunophenotypic and molecular characterization of mononuclear leukocytes (MNLs) in HCC patients treated with Transarterial chemoembolization (TACE).
METHODS: Serum and MNLs were collected from 20 TACE-treated HCC patients before (baseline-control samples) and after treatment with 5 g curcumin capsules , 10 mg piperine and 0.5 mg taurine taken daily for three consecutive months. Immunophenotypic and molecular characterization of MNLs were determined by flow cytometry and quantitative real time PCR, respectively. In addition, serum IFN-γ level was quantified by ELISA.
RESULTS: After receiving treatment with CPT combination, there was a highly significant increase in IFN- γ levels in the sera of patients when compared to basal line control samples. Additionally, the group receiving combined therapy demonstrated a downregulation in the expression levels of PD-1, in MNLs as compared to controls. MNLs\' immunophenotyping revealed a significant decline in CD4+CD25+cells (regulatory T lymphocytes). Furthermore, clinicopathological characteristics revealed a highly significant impact of CPT combination on aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and alpha feto protein (AFP) levels.
CONCLUSIONS: This study introduces a promising adjuvant CPT combined treatment as natural agents to enhance the management of HCC patients who are candidates to TACE treatment.
METHODS: Serum and MNLs were collected from 20 TACE-treated HCC patients before (baseline-control samples) and after treatment with 5 g curcumin capsules , 10 mg piperine and 0.5 mg taurine taken daily for three consecutive months. Immunophenotypic and molecular characterization of MNLs were determined by flow cytometry and quantitative real time PCR, respectively. In addition, serum IFN-γ level was quantified by ELISA.
RESULTS: After receiving treatment with CPT combination, there was a highly significant increase in IFN- γ levels in the sera of patients when compared to basal line control samples. Additionally, the group receiving combined therapy demonstrated a downregulation in the expression levels of PD-1, in MNLs as compared to controls. MNLs\' immunophenotyping revealed a significant decline in CD4+CD25+cells (regulatory T lymphocytes). Furthermore, clinicopathological characteristics revealed a highly significant impact of CPT combination on aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and alpha feto protein (AFP) levels.
CONCLUSIONS: This study introduces a promising adjuvant CPT combined treatment as natural agents to enhance the management of HCC patients who are candidates to TACE treatment.
摘要：
背景：大多数肝细胞癌（HCC）患者的诊断发生在中晚期，有一些治愈性的治疗选择。因此，迫切需要发现针对这种致命恶性肿瘤的其他辅助疗法。本研究旨在评估姜黄素（C）的有效性，胡椒碱（P）和牛磺酸（T）组合作为血清IFN-γ水平的佐剂，经动脉化疗栓塞（TACE）治疗的HCC患者中单核白细胞（MNLs）的免疫表型和分子特征。
方法：在用5g姜黄素胶囊治疗之前（基线对照样品）和之后，从20例TACE治疗的HCC患者中收集血清和MNLs，每天服用10mg胡椒碱和0.5mg牛磺酸，连续三个月。通过流式细胞术和实时定量PCR测定MNL的免疫表型和分子特征。分别。此外,通过ELISA定量血清IFN-γ水平。
结果：接受CPT联合治疗后，与基线对照样品相比，患者血清中的IFN-γ水平显着增加。此外,与对照组相比,接受联合治疗组的MNL中PD-1表达水平下调.MNLs免疫表型分析显示CD4+CD25+细胞(调节性T淋巴细胞)显著下降。此外，临床病理特征显示CPT组合对天冬氨酸转氨酶（AST）有非常显著的影响，乳酸脱氢酶（LDH）和α-feto蛋白（AFP）水平。
结论：本研究引入了一种有希望的辅助CPT联合治疗作为天然药物，以提高TACE治疗候选HCC患者的管理。
方法：在用5g姜黄素胶囊治疗之前（基线对照样品）和之后，从20例TACE治疗的HCC患者中收集血清和MNLs，每天服用10mg胡椒碱和0.5mg牛磺酸，连续三个月。通过流式细胞术和实时定量PCR测定MNL的免疫表型和分子特征。分别。此外,通过ELISA定量血清IFN-γ水平。
结果：接受CPT联合治疗后，与基线对照样品相比，患者血清中的IFN-γ水平显着增加。此外,与对照组相比,接受联合治疗组的MNL中PD-1表达水平下调.MNLs免疫表型分析显示CD4+CD25+细胞(调节性T淋巴细胞)显著下降。此外，临床病理特征显示CPT组合对天冬氨酸转氨酶（AST）有非常显著的影响，乳酸脱氢酶（LDH）和α-feto蛋白（AFP）水平。
结论：本研究引入了一种有希望的辅助CPT联合治疗作为天然药物，以提高TACE治疗候选HCC患者的管理。
