Abstract：
BACKGROUND: Several recent studies suggest that chromodomain-helicase -DNA-binding domains (CHDs) are linked with cancers. We explored the association between chromodomain-Helicase-DNA-binding domain proteins and breast cancer (BrCa) and introduced potential prognostic markers using various databases.
METHODS: We analyzed the expression of the CHD family and their prognostic value in BrCa by mining UALCAN, TIMER, and Kaplan-Meier plotter databases. The association of CHD expression and immune infiltrating abundance was studied via the TIMER database. In addition, microRNAs related to the CHD family were identified by using the MirTarBase online database.
RESULTS: The present study indicated that compared to normal tissues, BrCa tissues showed increased mRNA levels of CHD3/4/7 but decreased CHD2/5/9 expression. Interestingly, We also found a positive correlation between CHD gene expression and the infiltration of macrophage, neutrophil, and dendritic cells in BrCa, except CHD3/5. The Kaplan-Meier Plotter analysis suggested that high expression levels of CHD1/2/3/4/6/8/9 were significantly related to shorter relapse-free survival (RFS), while higher mRNA expression of CHD1, CHD2, CHD8, and CHD9 was significantly associated with longer overall survival of BrCa patients. The miRNAs of hsa-miR-615-3p and hsa-let-7b-5p were identified as being more correlated with the CHD family.
CONCLUSIONS: The altered expression of some CHD members was significantly related to clinical cancer outcomes, and CHD1/2/8/9 could serve as potential prognostic biomarkers to improve the survival of BrCa patients. However, to evaluate the studied CHD members in detail are needed further investigations including experimental validation.
METHODS: We analyzed the expression of the CHD family and their prognostic value in BrCa by mining UALCAN, TIMER, and Kaplan-Meier plotter databases. The association of CHD expression and immune infiltrating abundance was studied via the TIMER database. In addition, microRNAs related to the CHD family were identified by using the MirTarBase online database.
RESULTS: The present study indicated that compared to normal tissues, BrCa tissues showed increased mRNA levels of CHD3/4/7 but decreased CHD2/5/9 expression. Interestingly, We also found a positive correlation between CHD gene expression and the infiltration of macrophage, neutrophil, and dendritic cells in BrCa, except CHD3/5. The Kaplan-Meier Plotter analysis suggested that high expression levels of CHD1/2/3/4/6/8/9 were significantly related to shorter relapse-free survival (RFS), while higher mRNA expression of CHD1, CHD2, CHD8, and CHD9 was significantly associated with longer overall survival of BrCa patients. The miRNAs of hsa-miR-615-3p and hsa-let-7b-5p were identified as being more correlated with the CHD family.
CONCLUSIONS: The altered expression of some CHD members was significantly related to clinical cancer outcomes, and CHD1/2/8/9 could serve as potential prognostic biomarkers to improve the survival of BrCa patients. However, to evaluate the studied CHD members in detail are needed further investigations including experimental validation.
摘要：
背景：最近的几项研究表明，色域-解旋酶-DNA结合域（CHDs）与癌症有关。我们探索了色域-解旋酶-DNA结合域蛋白与乳腺癌（BrCa）之间的关联，并使用各种数据库介绍了潜在的预后标志物。
方法：我们通过挖掘UALCAN分析了CHD家族在BrCa中的表达及其预后价值，TIMER,和Kaplan-Meier绘图仪数据库。通过TIMER数据库研究了CHD表达和免疫浸润丰度的关联。此外,通过使用MirTarBase在线数据库鉴定与CHD家族相关的微小RNA。
结果：本研究表明，与正常组织相比，BrCa组织显示CHD3/4/7的mRNA水平升高，但CHD2/5/9的表达降低。有趣的是,我们还发现CHD基因表达与巨噬细胞浸润呈正相关,中性粒细胞,和BrCa中的树突状细胞，除CHD3/5。Kaplan-MeierPlotter分析显示，CHD1/2/3/4/6/8/9的高表达水平与较短的无复发生存期（RFS）显着相关。而CHD1，CHD2，CHD8和CHD9的mRNA表达较高与BrCa患者的总生存期较长显著相关。hsa-miR-615-3p和hsa-let-7b-5p的miRNA被鉴定为与CHD家族更相关。
结论：一些CHD成员的表达改变与临床癌症预后显著相关，和CHD1/2/8/9可以作为潜在的预后生物标志物，以提高BrCa患者的生存率。然而,为了详细评估所研究的CHD成员，需要进一步的研究，包括实验验证。
方法：我们通过挖掘UALCAN分析了CHD家族在BrCa中的表达及其预后价值，TIMER,和Kaplan-Meier绘图仪数据库。通过TIMER数据库研究了CHD表达和免疫浸润丰度的关联。此外,通过使用MirTarBase在线数据库鉴定与CHD家族相关的微小RNA。
结果：本研究表明，与正常组织相比，BrCa组织显示CHD3/4/7的mRNA水平升高，但CHD2/5/9的表达降低。有趣的是,我们还发现CHD基因表达与巨噬细胞浸润呈正相关,中性粒细胞,和BrCa中的树突状细胞，除CHD3/5。Kaplan-MeierPlotter分析显示，CHD1/2/3/4/6/8/9的高表达水平与较短的无复发生存期（RFS）显着相关。而CHD1，CHD2，CHD8和CHD9的mRNA表达较高与BrCa患者的总生存期较长显著相关。hsa-miR-615-3p和hsa-let-7b-5p的miRNA被鉴定为与CHD家族更相关。
结论：一些CHD成员的表达改变与临床癌症预后显著相关，和CHD1/2/8/9可以作为潜在的预后生物标志物，以提高BrCa患者的生存率。然而,为了详细评估所研究的CHD成员，需要进一步的研究，包括实验验证。
