Abstract：
The exceptional biocompatibility and adaptability of hydrogels have garnered significant interest in the biomedical field for the fabrication of biomedical devices. However, conventional synthetic hydrogels still exhibit relatively weak and fragile properties. Drawing inspiration from the photosynthesis process, we developed a facile approach to achieve a harmonious combination of superior mechanical properties and efficient preparation of silk fibroin hydrogel through photo-cross-linking technology, accomplished within 60 s. The utilization of riboflavin and H2O2 enabled a sustainable cyclic photo-cross-linking reaction, facilitating the transformation from tyrosine to dityrosine and ultimately contributing to the formation of highly cross-linked hydrogels. These photo-cross-linking hydrogels exhibited excellent elasticity and restorability even after undergoing 1000 cycles of compression. Importantly, our findings presented that hydrogel-encapsulated adipose stem cells possess the ability to stimulate cell proliferation along with stem cell stemness. This was evidenced by the continuous high expression levels of OCT4 and SOX2 over 21 days. Additionally, the utilization of photo-cross-linking hydrogels can be extended to various material molding platforms, including microneedles, microcarriers, and bone screws. Consequently, this study offered a significant approach to fabricating biomedical hydrogels capable of facilitating real-time cell delivery, thereby introducing an innovative avenue for designing silk devices with exceptional machinability and adaptability in biomedical applications.
摘要：
水凝胶的优异的生物相容性和适应性已经在用于制造生物医学装置的生物医学领域中引起了极大的兴趣。然而,传统的合成水凝胶仍然表现出相对较弱和易碎的性质。从光合作用过程中汲取灵感，我们开发了一种简单的方法，通过光交联技术实现了卓越的机械性能和高效制备丝素蛋白水凝胶的和谐结合，在60s内完成。核黄素和H2O2的利用实现了可持续的循环光交联反应，促进从酪氨酸到二酪氨酸的转化，并最终有助于形成高度交联的水凝胶。这些光交联水凝胶即使在经历1000次压缩循环后也表现出优异的弹性和恢复性。重要的是,我们的研究结果表明,水凝胶包裹的脂肪干细胞具有刺激细胞增殖和干细胞干细胞干细胞的能力.这通过在21天内OCT4和S0X2的连续高表达水平来证明。此外,光交联水凝胶的利用可以扩展到各种材料成型平台，包括微针,微载体,和骨螺钉。因此,这项研究提供了一种制造能够促进实时细胞递送的生物医学水凝胶的重要方法，从而为设计在生物医学应用中具有特殊机械加工性和适应性的丝绸设备引入了创新途径。
