Abstract：
OBJECTIVE: The aim of the present study was to assess the cytocompatibility of epoxy resin-based AH Plus Jet (Dentsply De Trey, Konstanz, Germany), Sealer Plus (MK Life, Porto Alegre, Brazil), calcium silicate-based Bio-C Sealer (Angelus, Londrina, PR, Brazil), Sealer Plus BC (MK Life) and AH Plus BC (Dentsply) through a tridimensional (3D) culture model of human osteoblast-like cells.
METHODS: Spheroids of MG-63 cells were produced and exposed to fresh root canal sealers extracts by 24 h, and the cytotoxicity was assessed by the Lactate Dehydrogenase assay (LDH). The distribution of dead cells within the microtissue was assessed by fluorescence microscopy, and morphological effects were investigated by histological analysis. The secreted inflammatory mediators were detected in cell supernatants through flow luminometry (XMap Luminex).
RESULTS: Cells incubated with AH Plus Jet, AH Plus BC, Sealer Plus BC and Bio-C Sealer extracts showed high rates of cell viability, while the Sealer Plus induced a significant reduction of cell viability, causing reduction on the spheroid structure. Sealer Plus and Seaker Plus BC caused alterations on 3D microtissue morphology. The AH Plus BC extract was associated with the downregulation of secretion of pro-inflammatory cytokines IL-5, IL-7, IP-10 and RANTES.
CONCLUSIONS: The new AH Plus BC calcium silicate-based endodontic sealer did not reduce cell viability in vitro, while led to the downregulation of pro-inflammatory cytokines.
CONCLUSIONS: Choosing the appropriate endodontic sealer is a crucial step. AH Plus BC demonstrated high cell viability and downregulation of pro-inflammatory cytokines, appearing reliable for clinical use, while Sealer Plus presented lower cytocompatibility.
METHODS: Spheroids of MG-63 cells were produced and exposed to fresh root canal sealers extracts by 24 h, and the cytotoxicity was assessed by the Lactate Dehydrogenase assay (LDH). The distribution of dead cells within the microtissue was assessed by fluorescence microscopy, and morphological effects were investigated by histological analysis. The secreted inflammatory mediators were detected in cell supernatants through flow luminometry (XMap Luminex).
RESULTS: Cells incubated with AH Plus Jet, AH Plus BC, Sealer Plus BC and Bio-C Sealer extracts showed high rates of cell viability, while the Sealer Plus induced a significant reduction of cell viability, causing reduction on the spheroid structure. Sealer Plus and Seaker Plus BC caused alterations on 3D microtissue morphology. The AH Plus BC extract was associated with the downregulation of secretion of pro-inflammatory cytokines IL-5, IL-7, IP-10 and RANTES.
CONCLUSIONS: The new AH Plus BC calcium silicate-based endodontic sealer did not reduce cell viability in vitro, while led to the downregulation of pro-inflammatory cytokines.
CONCLUSIONS: Choosing the appropriate endodontic sealer is a crucial step. AH Plus BC demonstrated high cell viability and downregulation of pro-inflammatory cytokines, appearing reliable for clinical use, while Sealer Plus presented lower cytocompatibility.
摘要：
目的：本研究的目的是评估基于环氧树脂的AHPlusJet的细胞相容性（DentsplyDeTrey，康斯坦茨,德国),SealerPlus(MKLife,阿雷格里港,巴西),硅酸钙基Bio-C密封剂(Angelus，Londrina,PR,巴西),SealerPlusBC（MKLife）和AHPlusBC（Dentsply）通过人成骨细胞样细胞的三维（3D）培养模型。
方法：产生MG-63细胞的球体，并暴露于新鲜的根管封闭剂提取物24小时，并通过乳酸脱氢酶测定(LDH)评估细胞毒性。通过荧光显微镜评估死细胞在显微组织内的分布，并通过组织学分析研究了形态学影响。通过流动发光法(XMappLuminex)在细胞上清液中检测分泌的炎症介质。
结果：用AHPlusJet孵育的细胞，啊加上BC，SealerPlusBC和Bio-CSealer提取物显示出很高的细胞活力，而SealerPlus诱导细胞活力显著降低，导致球状体结构减少。SealerPlus和SeakerPlusBC引起了3D微组织形态的改变。AH加BC提取物与促炎细胞因子IL-5、IL-7、IP-10和RANTES的分泌下调有关。
结论：新的基于AHPlusBC硅酸钙的牙髓封闭剂在体外没有降低细胞活力，而导致促炎细胞因子的下调。
结论：选择合适的牙髓封闭剂是至关重要的一步。AHPlusBC表现出高细胞活力和促炎细胞因子的下调，看起来可靠的临床使用，而SealerPlus的细胞相容性较低。
方法：产生MG-63细胞的球体，并暴露于新鲜的根管封闭剂提取物24小时，并通过乳酸脱氢酶测定(LDH)评估细胞毒性。通过荧光显微镜评估死细胞在显微组织内的分布，并通过组织学分析研究了形态学影响。通过流动发光法(XMappLuminex)在细胞上清液中检测分泌的炎症介质。
结果：用AHPlusJet孵育的细胞，啊加上BC，SealerPlusBC和Bio-CSealer提取物显示出很高的细胞活力，而SealerPlus诱导细胞活力显著降低，导致球状体结构减少。SealerPlus和SeakerPlusBC引起了3D微组织形态的改变。AH加BC提取物与促炎细胞因子IL-5、IL-7、IP-10和RANTES的分泌下调有关。
结论：新的基于AHPlusBC硅酸钙的牙髓封闭剂在体外没有降低细胞活力，而导致促炎细胞因子的下调。
结论：选择合适的牙髓封闭剂是至关重要的一步。AHPlusBC表现出高细胞活力和促炎细胞因子的下调，看起来可靠的临床使用，而SealerPlus的细胞相容性较低。
