PDF(Pubmed)
Abstract：
The Ningxiang pig, a distinguished local breed in China, is recognized for its good meat quality traits. This study examines the proteomics of Ningxiang pigs at three developmental stages and delves into the upstream transcriptomics of these proteomics. Such an analysis facilitates a deeper understanding of the molecular interplay between proteins and transcriptomes in the Ningxiang pig muscle, influencing muscle growth and development. In this research, we analyzed the muscles of Ningxiang pigs at three developmental stages: 30 days in weaned piglets, 90 days in nursery pigs, and 210 days in late fattening pigs. There a total of 16 differentially co-expressed miRNAs (ssc-miRNA-1, ssc-miRNA-378, ssc-miRNA-143, ssc-miRNA-30e, etc.), 74 differentially co-expressed mRNA (PLIN3, CPT2, IGF2 and HSP90AB1, etc.) have been identified in the three stages. 572 differentially abundant proteins (DAPs) (APOC3, NDUFA2, HSPD1, ATP5E, PDHA1, etc.) were readily identified by comparing different time periods. According to the KEGG enrich pathway results that DAPs most enriched in growth and development pathways, immune mechanism pathways and maintaining functions of physical. Through short time-series expression miner (STEM) association analysis, a total of 571 negative miRNA-mRNA interaction pairs and 2 negative miRNA-mRNA-protein (Chr05_11955-Pig.17268.1-ATP5F1B, ssc-miR-194a-3p-Pig.15802.1-ACY1) interaction pairs were found. Our study provides a theoretical basis on molecular mechanism for the study of IMF deposition, muscle growth and immunity in Ningxiang pig breed.
摘要：
宁乡猪，中国的一个杰出的地方品种，以其良好的肉质性状而闻名。本研究考察了宁乡猪在三个发育阶段的蛋白质组学，并深入研究了这些蛋白质组学的上游转录组学。这样的分析有助于更深入地了解宁乡猪肌肉中蛋白质和转录组之间的分子相互作用，影响肌肉生长和发育。在这项研究中,我们分析了宁乡猪三个发育阶段的肌肉：断奶仔猪30天，在保育猪身上90天,和晚育猪210天。共有16种差异共表达的miRNA(ssc-miRNA-1,ssc-miRNA-378,ssc-miRNA-143,ssc-miRNA-30e,等。),74差异共表达的mRNA（PLIN3，CPT2，IGF2和HSP90AB1等。)已在三个阶段中确定。572个差异丰富蛋白（DAP）（APOC3，NDUFA2，HSPD1，ATP5E，PDHA1等.)很容易通过比较不同的时间段来识别。根据KEGG富集途径的结果，DAP在生长和发育途径中最富集，免疫机制途径和维持身体功能。通过短时序列表达矿工(STEM)关联分析，共571个阴性miRNA-mRNA相互作用对和2个阴性miRNA-mRNA-蛋白（Chr05_11955-Pig.17268.1-ATP5F1B，发现ssc-miR-194a-3p-Pig.15802.1-ACY1)相互作用对。本研究为IMF沉积的分子机制研究提供了理论依据,宁乡猪的肌肉生长和免疫力。
