PDF(Pubmed)
Abstract：
Acorn barnacles are efficient colonizers on a wide variety of marine surfaces. As they proliferate on critical infrastructure, their settlement and growth have deleterious effects on performance. To address acorn barnacle biofouling, research has focused on the settlement and adhesion processes with the goal of informing the development of novel coatings. This effort has resulted in the discovery and characterization of several proteins found at the adhesive substrate interface, i.e. cement proteins, and a deepened understanding of the function and composition of the biomaterials within this region. While the adhesive properties at the interface are affected by the interaction between the proteins, substrate and mechanics of the calcified base plate, little attention has been given to the interaction between the proteins and the cuticular material present at the substrate interface. Here, the proteome of the organic matrix isolated from the base plate of the acorn barnacle Amphibalanus amphitrite is compared with the chitinous and proteinaceous matrix embedded within A. amphitrite parietal plates. The objective was to gain an understanding of how the basal organic matrix may be specialized for adhesion via an in-depth comparative proteome analysis. In general, the majority of proteins identified in the parietal matrix were also found in the basal organic matrix, including nearly all those grouped in classes of cement proteins, enzymes and pheromones. However, the parietal organic matrix was enriched with cuticle-associated proteins, of which ca 30% of those identified were unique to the parietal region. In contrast, ca 30-40% of the protease inhibitors, enzymes and pheromones identified in the basal organic matrix were unique to this region. Not unexpectedly, nearly 50% of the cement proteins identified in the basal region were significantly distinct from those found in the parietal region. The wider variety of identified proteins in the basal organic matrix indicates a greater diversity of biological function in the vicinity of the substrate interface where several processes related to adhesion, cuticle formation and expansion of the base synchronize to play a key role in organism survival.
摘要：
橡子藤壶是各种海洋表面上的有效定殖者。随着它们在关键基础设施上的扩散，他们的定居和成长对业绩有有害影响。为了解决橡子藤壶生物污染，研究的重点是沉降和粘附过程，目的是为新型涂料的开发提供信息。这项努力导致了在粘合剂基底界面发现的几种蛋白质的发现和表征。即水泥蛋白，并加深了对该地区生物材料的功能和组成的了解。虽然界面的粘附特性受到蛋白质之间相互作用的影响，钙化底板的基底和力学，很少注意蛋白质和基质界面上存在的角质层物质之间的相互作用。这里,将从橡子藤壶两栖动物基板中分离出的有机基质的蛋白质组与嵌入在A.Aphitrite顶板中的几丁质和蛋白质基质进行比较。目的是通过深入的比较蛋白质组分析，了解如何将基础有机基质专门用于粘附。总的来说,在顶叶基质中鉴定的大多数蛋白质也在基础有机基质中发现，包括几乎所有按水泥蛋白分类的蛋白质，酶和信息素。然而,顶叶有机基质富含角质层相关蛋白，其中约30%的鉴定是顶叶区域特有的。相比之下,大约30-40%的蛋白酶抑制剂，在基础有机基质中鉴定出的酶和信息素是该区域特有的。不出所料,在基底区域发现的近50％的水泥蛋白与在顶叶区域发现的那些明显不同。在基础有机基质中鉴定出的蛋白质种类繁多，表明在与粘附有关的几个过程的底物界面附近，生物功能具有更大的多样性。角质层的形成和基部的扩张同步在生物体的生存中起着关键作用。
