Abstract：
Lumpy Skin Disease (LSD), a poxvirus disease affecting cattle, emerged in India in 2019 and intensified in 2022, resulting in significant economic losses for dairy farmers. There was unusual shift in mortality and morbidity patterns during the second wave. A comprehensive genetic study conducted, analyzing samples from 2019 to 2022 revealed circulation of two distinct subclades (subclade 1.2a and 1.2b) in India, with the latter showing a different pattern in morbidity and mortality. Notably, the Ankyrin repeats gene-based analysis could differentiate animals with varying clinical scores. Genetic variations were significant, with unique deletions identified, including a 12-nucleotide deletion in the GPCR gene in virus isolates collected during 2022 outbreaks, not reported earlier in Indian LSDV strains. A crucial finding was a significant 95-nucleotide deletion in the Functional Resolution Sequence (FRS) repeats of LSDV genomes from 2022 outbreaks, absent in 2019 samples. These deletions may have influenced the virus\'s virulence in India.
摘要：
块状皮肤病（LSD），一种影响牛的痘病毒病，2019年在印度出现，2022年愈演愈烈，给奶农造成了巨大的经济损失。在第二波中，死亡率和发病率模式发生了异常变化。进行了一项全面的遗传研究，分析2019年至2022年的样本显示，印度有两个不同的子进化枝(1.2a和1.2b)，后者在发病率和死亡率方面表现出不同的模式。值得注意的是,基于Ankyrin重复基因的分析可以区分具有不同临床评分的动物.遗传变异显著，确定了唯一的删除，包括在2022年爆发期间收集的病毒分离株中GPCR基因的12个核苷酸缺失，印度LSDV菌株没有早期报道。一个重要的发现是2022年爆发的LSDV基因组的功能分辨率序列（FRS）重复序列中的95个核苷酸的显着缺失。2019年样本中没有。这些缺失可能影响了病毒在印度的毒力。
