Abstract：
BACKGROUND: Obstructive sleep apnea (OSA) is one of the risk factors for diabetes mellitus type 2 (DM2). As OSA is associated with the disruption of the circadian rhythm, it affects circadian clock proteins, including neuronal PAS domain protein 2 (NPAS2) and nuclear receptor subfamily 1 group D member 1 (Rev-Erb-α). These proteins have been shown to be related to metabolic abnormalities, i.a., insulin resistance.
OBJECTIVE: The present pilot study aimed to investigate the NPAS2 and Rev-Erb-α protein serum levels in the groups of patients with severe OSA and severe OSA+DM2 in comparison with healthy controls, taking into account correlations with polysomnography (PSG) parameters (e.g., oxygen saturation (SpO2) variables).
METHODS: A total of 40 participants were included in the study. They were split into 3 groups as follows: the OSA group (n = 17; apnea-hypopnea index (AHI) >30, no DM2); the OSA+DM2 group (n = 7; AHI > 30 and DM2); and the control group (n = 16; AHI < 5, no DM2). All participants underwent a nocturnal PSG examination and had their blood collected the following morning. The serum levels of NPAS2 and Rev-Erb-α proteins were assessed using the enzyme-linked immunosorbent assay (ELISA).
RESULTS: The mean NPAS2 protein level was significantly lower in the OSA group as compared to healthy individuals (p = 0.017). Additionally, the OSA group presented with lower NPAS2 protein levels as compared to the OSA+DM2 group, but only a tendency was observed (p = 0.094). No differences in the Rev-Erb-α protein concentration were noticed. Furthermore, a negative correlation between AHI during rapid eye movement (REM) sleep and the NPAS2 protein serum level was observed (r = -0.478; p = 0.002).
CONCLUSIONS: Serum NPAS2 protein might be involved in metabolic dysregulation present among OSA patients, while the mechanism itself may be associated with REM sleep.
OBJECTIVE: The present pilot study aimed to investigate the NPAS2 and Rev-Erb-α protein serum levels in the groups of patients with severe OSA and severe OSA+DM2 in comparison with healthy controls, taking into account correlations with polysomnography (PSG) parameters (e.g., oxygen saturation (SpO2) variables).
METHODS: A total of 40 participants were included in the study. They were split into 3 groups as follows: the OSA group (n = 17; apnea-hypopnea index (AHI) >30, no DM2); the OSA+DM2 group (n = 7; AHI > 30 and DM2); and the control group (n = 16; AHI < 5, no DM2). All participants underwent a nocturnal PSG examination and had their blood collected the following morning. The serum levels of NPAS2 and Rev-Erb-α proteins were assessed using the enzyme-linked immunosorbent assay (ELISA).
RESULTS: The mean NPAS2 protein level was significantly lower in the OSA group as compared to healthy individuals (p = 0.017). Additionally, the OSA group presented with lower NPAS2 protein levels as compared to the OSA+DM2 group, but only a tendency was observed (p = 0.094). No differences in the Rev-Erb-α protein concentration were noticed. Furthermore, a negative correlation between AHI during rapid eye movement (REM) sleep and the NPAS2 protein serum level was observed (r = -0.478; p = 0.002).
CONCLUSIONS: Serum NPAS2 protein might be involved in metabolic dysregulation present among OSA patients, while the mechanism itself may be associated with REM sleep.
摘要：
背景：阻塞性睡眠呼吸暂停（OSA）是2型糖尿病（DM2）的危险因素之一。由于OSA与昼夜节律的破坏有关，它会影响生物钟蛋白质，包括神经元PAS结构域蛋白2(NPAS2)和核受体亚家族1组D成员1(Rev-Erb-α)。这些蛋白质已被证明与代谢异常有关，i.a.,胰岛素抵抗。
目的：本试验研究旨在研究重度OSA和重度OSA+DM2患者与健康对照组比较的NPAS2和Rev-Erb-α蛋白血清水平。考虑到与多导睡眠图(PSG)参数的相关性(例如，氧饱和度（SpO2）变量）。
方法：共有40名参与者被纳入研究。分为3组：OSA组（n=17；呼吸暂停低通气指数（AHI）>30，无DM2）；OSADM2组（n=7；AHI>30和DM2）；和对照组（n=16；AHI<5，无DM2）。所有参与者都接受了夜间PSG检查，并在第二天早上收集了血液。使用酶联免疫吸附测定（ELISA）评估NPAS2和Rev-Erb-α蛋白的血清水平。
结果：与健康个体相比，OSA组的平均NPAS2蛋白水平显着降低（p=0.017）。此外,与OSA+DM2组相比，OSA组的NPAS2蛋白水平较低，但只观察到一种趋势(p=0.094)。未观察到Rev-Erb-α蛋白浓度的差异。此外，观察到快速眼动（REM）睡眠期间的AHI与NPAS2蛋白血清水平之间呈负相关（r=-0.478；p=0.002）。
结论：血清NPAS2蛋白可能参与OSA患者的代谢失调，而机制本身可能与REM睡眠有关。
目的：本试验研究旨在研究重度OSA和重度OSA+DM2患者与健康对照组比较的NPAS2和Rev-Erb-α蛋白血清水平。考虑到与多导睡眠图(PSG)参数的相关性(例如，氧饱和度（SpO2）变量）。
方法：共有40名参与者被纳入研究。分为3组：OSA组（n=17；呼吸暂停低通气指数（AHI）>30，无DM2）；OSADM2组（n=7；AHI>30和DM2）；和对照组（n=16；AHI<5，无DM2）。所有参与者都接受了夜间PSG检查，并在第二天早上收集了血液。使用酶联免疫吸附测定（ELISA）评估NPAS2和Rev-Erb-α蛋白的血清水平。
结果：与健康个体相比，OSA组的平均NPAS2蛋白水平显着降低（p=0.017）。此外,与OSA+DM2组相比，OSA组的NPAS2蛋白水平较低，但只观察到一种趋势(p=0.094)。未观察到Rev-Erb-α蛋白浓度的差异。此外，观察到快速眼动（REM）睡眠期间的AHI与NPAS2蛋白血清水平之间呈负相关（r=-0.478；p=0.002）。
结论：血清NPAS2蛋白可能参与OSA患者的代谢失调，而机制本身可能与REM睡眠有关。
