PDF(Pubmed)
Abstract：
UNASSIGNED: This study aimed to explore the effects of removable aligners and fixed appliances on the supragingival bacterial communities in adult female patients undergoing orthodontic treatment.
UNASSIGNED: Supragingival plaque samples from 48 female individuals underwent microbiome analysis (16S rRNA gene sequencing) using PacBio Sequel sequencing. The study included 13 adults without orthodontic treatment needs as the control group (Group C), and 35 patients with comparable initial orthodontic conditions who received treatment at a university clinic in Beijing, China. The treatment involved either traditional fixed brackets (Group B, n = 17) or Invisalign® aligners (Group AT, n = 18). Bioinformatics methods were used for data analysis.
UNASSIGNED: From the 48 plaque samples, a total of 334,961 valid reads were obtained, averaging 6,978 sequences per sample. The 16S rDNA sequences were classified into 25,727 amplicon sequence variants (ASVs). Significant variances in alpha and beta diversity among the groups were noted. Group B microbiome exhibited an increased presence of Gram-negative bacteria. At the phylum level, Actinobacteriota was significantly more prevalent in Group C samples, while Bacteroidota was enriched in Group B samples. Family-level relative abundance analysis showed a notable increase in Saccharibacteria (formerly TM7) and Prevotellaceae in Group B. Genus-level analysis revealed a significant rise in Lautropia in Group AT. Fixed orthodontic appliances were linked to oral microbiome changes, notably an enhanced relative abundance of anaerobes, including periodontal pathogens.
UNASSIGNED: The observation points to the impact of orthodontic appliance on the oral microbial community, highlighting the difference between traditional braces (Group B) and clear aligners (Group AT)in terms of the predominance of anaerobic and gram negative bacteria. This emphasizes the importance of considering the microbiological effects when choosing orthodontic appliance and underscores the need for tailored oral hygiene practices for individuals undergoing these treatments. This research might provide insights that could assist in the development of innovative cleaning techniques and antibacterial materials.
UNASSIGNED: Supragingival plaque samples from 48 female individuals underwent microbiome analysis (16S rRNA gene sequencing) using PacBio Sequel sequencing. The study included 13 adults without orthodontic treatment needs as the control group (Group C), and 35 patients with comparable initial orthodontic conditions who received treatment at a university clinic in Beijing, China. The treatment involved either traditional fixed brackets (Group B, n = 17) or Invisalign® aligners (Group AT, n = 18). Bioinformatics methods were used for data analysis.
UNASSIGNED: From the 48 plaque samples, a total of 334,961 valid reads were obtained, averaging 6,978 sequences per sample. The 16S rDNA sequences were classified into 25,727 amplicon sequence variants (ASVs). Significant variances in alpha and beta diversity among the groups were noted. Group B microbiome exhibited an increased presence of Gram-negative bacteria. At the phylum level, Actinobacteriota was significantly more prevalent in Group C samples, while Bacteroidota was enriched in Group B samples. Family-level relative abundance analysis showed a notable increase in Saccharibacteria (formerly TM7) and Prevotellaceae in Group B. Genus-level analysis revealed a significant rise in Lautropia in Group AT. Fixed orthodontic appliances were linked to oral microbiome changes, notably an enhanced relative abundance of anaerobes, including periodontal pathogens.
UNASSIGNED: The observation points to the impact of orthodontic appliance on the oral microbial community, highlighting the difference between traditional braces (Group B) and clear aligners (Group AT)in terms of the predominance of anaerobic and gram negative bacteria. This emphasizes the importance of considering the microbiological effects when choosing orthodontic appliance and underscores the need for tailored oral hygiene practices for individuals undergoing these treatments. This research might provide insights that could assist in the development of innovative cleaning techniques and antibacterial materials.
摘要：
■本研究旨在探讨可移动矫正器和固定矫正器对接受正畸治疗的成年女性患者牙龈上细菌群落的影响。
■使用PacBioSequel测序对来自48名女性个体的牙龈上菌斑样本进行了微生物组分析（16SrRNA基因测序）。该研究包括13名没有正畸治疗需要的成年人作为对照组（C组），和35名在北京一所大学诊所接受治疗的初始正畸条件相当的患者，中国。治疗包括传统的固定支架(B组，n=17)或Invisalign®对准器(AT组，n=18)。采用生物信息学方法进行数据分析。
■从48个菌斑样本中，总共获得了334,961个有效读数，平均每个样本6,978个序列。16SrDNA序列被分类为25,727个扩增子序列变体(ASV)。注意到各组之间α和β多样性的显着差异。B组微生物组显示革兰氏阴性细菌的存在增加。在门一级，放线菌在C组样本中明显更为普遍，而B组样本中富含拟杆菌。家庭水平的相对丰度分析显示，B组的糖胞菌（以前为TM7）和Prevotellaceae显着增加。属水平分析显示，AT组的Lautropia显着增加。固定正畸矫治器与口腔微生物组变化有关，尤其是相对丰富的厌氧菌，包括牙周病原体。
■观察指出正畸矫治器对口腔微生物群落的影响，突出了传统牙套（B组）和清晰对齐（AT组）在厌氧和革兰氏阴性菌优势方面的差异。这强调了在选择正畸矫治器时考虑微生物学效应的重要性，并强调了需要为接受这些治疗的个体量身定制的口腔卫生实践。这项研究可能提供见解，可以帮助开发创新的清洁技术和抗菌材料。
■使用PacBioSequel测序对来自48名女性个体的牙龈上菌斑样本进行了微生物组分析（16SrRNA基因测序）。该研究包括13名没有正畸治疗需要的成年人作为对照组（C组），和35名在北京一所大学诊所接受治疗的初始正畸条件相当的患者，中国。治疗包括传统的固定支架(B组，n=17)或Invisalign®对准器(AT组，n=18)。采用生物信息学方法进行数据分析。
■从48个菌斑样本中，总共获得了334,961个有效读数，平均每个样本6,978个序列。16SrDNA序列被分类为25,727个扩增子序列变体(ASV)。注意到各组之间α和β多样性的显着差异。B组微生物组显示革兰氏阴性细菌的存在增加。在门一级，放线菌在C组样本中明显更为普遍，而B组样本中富含拟杆菌。家庭水平的相对丰度分析显示，B组的糖胞菌（以前为TM7）和Prevotellaceae显着增加。属水平分析显示，AT组的Lautropia显着增加。固定正畸矫治器与口腔微生物组变化有关，尤其是相对丰富的厌氧菌，包括牙周病原体。
■观察指出正畸矫治器对口腔微生物群落的影响，突出了传统牙套（B组）和清晰对齐（AT组）在厌氧和革兰氏阴性菌优势方面的差异。这强调了在选择正畸矫治器时考虑微生物学效应的重要性，并强调了需要为接受这些治疗的个体量身定制的口腔卫生实践。这项研究可能提供见解，可以帮助开发创新的清洁技术和抗菌材料。
