Abstract：
This study synthesized zinc oxide nanoparticles (ZnO NPs) using a novel green approach, with Sida acuta leaf extract as a capping and reducing agent to initiate nucleation and structure formation. The innovation of this study lies in demonstrating the originality of utilizing zinc oxide nanoparticles for antibacterial action, antioxidant potential, and catalytic degradation of Congo red dye. This unique approach harnesses eco-friendly methods to initiate nucleation and structure formation. The synthesized nanoparticles\' structure and conformation were characterized using UV-vis (λmax = 280 nm), X-ray, atomic force microscopy, SEM, HR-TEM and FTIR. The antibacterial activity of the Nps was tested against Pseudomonas sp, Klebsiella sp, Staphylococcus aureus, and E. coli, demonstrating efficacy. The nanoparticles exhibited unique properties, with a crystallite size of 20 nm (XRD), a surface roughness of 2.5 nm (AFM), and a specific surface area of 60 m2/g (SEM). A Convolutional Neural Network (CNN) was effectively employed to accurately classify and analyze microscopic images of green-synthesized zinc oxide nanoparticles. This research revealed their exceptional antioxidant potential, with an average DPPH scavenging rate of 80% at a concentration of 0.05 mg/mL. Additionally, zeta potential measurements indicated a stable net negative surface charge of approximately -12.2 mV. These quantitative findings highlight the promising applications of green-synthesized ZnO NPs in healthcare, materials science, and environmental remediation. The ZnO nanoparticles exhibited catalytic capabilities for dye degradation, and the degradation rate was determined using UV spectroscopy. Key findings of the study encompass the green synthesis of versatile zinc oxide nanoparticles, demonstrating potent antibacterial action, antioxidant capabilities, and catalytic dye degradation potential. These nanoparticles offer multifaceted solutions with minimal environmental impact, addressing challenges in various fields, from healthcare to environmental remediation.
摘要：
本研究使用一种新颖的绿色方法合成了氧化锌纳米颗粒（ZnONPs），以四达叶提取物作为加帽和还原剂，引发成核和结构形成。本研究的创新之处在于证明了利用氧化锌纳米颗粒的抗菌作用的独创性，抗氧化潜力，和催化降解刚果红染料。这种独特的方法利用环保方法来引发成核和结构形成。使用UV-vis(λmax=280nm)表征合成的纳米颗粒的结构和构象,X光片,原子力显微镜,SEM,HR-TEM和FTIR。测试了Nps对假单胞菌的抗菌活性，克雷伯菌属,金黄色葡萄球菌,和大肠杆菌，展示功效。纳米粒子表现出独特的性质，微晶尺寸为20nm(XRD)，表面粗糙度为2.5nm（AFM），比表面积为60m2/g(SEM)。卷积神经网络（CNN）有效地用于精确分类和分析绿色合成的氧化锌纳米颗粒的显微图像。这项研究揭示了它们非凡的抗氧化潜力，浓度为0.05mg/mL时，DPPH的平均清除率为80％。此外,ζ电位测量表明大约-12.2mV的稳定净负表面电荷。这些定量发现突出了绿色合成ZnONPs在医疗保健中的有希望的应用，材料科学,和环境修复。ZnO纳米粒子表现出催化降解染料的能力，并使用紫外光谱法测定降解率。该研究的主要发现包括多功能氧化锌纳米颗粒的绿色合成，表现出有效的抗菌作用，抗氧化能力，和催化染料降解潜力。这些纳米粒子提供多方面的解决方案，对环境的影响最小，应对各个领域的挑战，从医疗保健到环境修复。
