Abstract：
Piroplasmosis, a tick-borne disease affecting livestock, including camels, is caused by intracellular apicomplexan parasites belonging to the order Piroplasmida. Despite its importance, there\'s limited research on piroplasmosis among Egyptian camels. This study aimed to fill this gap by investigating tick-borne piroplasmids in camels from Cairo and Giza Governorates. Out of 181 blood samples collected between October 2021 and March 2022 from apparently healthy one-humped camels (Camelus dromedarius), PCR assays revealed a 41.4 % infection rate with various piroplasmids. Detected species included B. bovis (17.7 %), B. bigemina (12.2 %), B. caballi (8.3 %), B. naoakii (11.6 %), B. microti (1.7 %), T. equi (4.4 %), and Theileria spp. (28.7 %). Phylogenetic analysis revealed the first detection of T. equi genotype E in Egypt and identified a novel B. caballi genotype. Additionally, B. microti isolates were identified as the US-type. These findings shed lights on piroplasmosis among Egyptian camels, and provide valuable information for devising effective control strategies, especially B. microti, a pathogen with potential human health risks.
摘要：
Piroplasmosis,一种影响牲畜的蜱传疾病，包括骆驼,是由属于Piroplasmida顺序的细胞内顶端丛寄生虫引起的。尽管它很重要,对埃及骆驼中的螺旋体病的研究有限。这项研究旨在通过调查开罗和吉萨省骆驼中的tick传播的piroplasmids来填补这一空白。在2021年10月至2022年3月期间从明显健康的单峰骆驼（Camelusdromedarius）收集的181个血液样本中，PCR检测显示，各种质粒的感染率为41.4％。检测到的物种包括牛芽孢杆菌(17.7%)，B.bigemina（12.2%），B.caballi（8.3%），B.Naoakii（11.6%），B.microti（1.7%），T、equi（4.4%），和Theileriaspp.（28.7%）。系统发育分析显示，在埃及首次检测到T.equi基因型E，并鉴定出一种新的B.caballi基因型。此外,B.microti分离株被鉴定为US型。这些发现揭示了埃及骆驼中的螺旋体病，并为设计有效的控制策略提供有价值的信息，尤其是B.microti,具有潜在人类健康风险的病原体。
