Abstract:
The purpose of this study was to characterize the activity of essential oils from Nepeta Cataria (EON) at concentrations of 125 μ L L-1, 150 μ L L-1, 175 μ L L-1, and 200 μ L L-1 on the behavior of loss of the posture reflex and recovery of the posture reflex and electrocardiographic activity and recording of the opercular beat of Colossoma macropomum during immersion bathing for a period of 5 min, in order to obtain a window for safe use during anesthesia. The fish (23.38 ± 3.5 g) were assigned to the following experiments: experiment 1 (latency to loss and recovery of the posture reflex): (a) 125 μ L L-1, (b) 150 μ L L-1, (c) 175 μ L L-1, and (d) 200 μ L L-1 (n = 9) per group. Experiment 2 (electrocardiographic and heartbeat recordings): (a) control group; (b) vehicle control group (2 ml of alcohol per liter of water), (c) 125 μ L L-1, (d) 150 μ L L-1, (e) 175 μ L L-1, and (f) 200 μ L L-1 (n = 9), per group. All the concentrations used showed efficacy in inducing loss of the posture reflex and reversibility with recovery of the posture reflex, but the electrocardiographic recordings indicated morphographic changes such as bradycardia during induction and p wave apiculation during recovery at the highest concentrations tested. In this way, we suggest a safe use window for short-term anesthesia with EON in the concentration range of 125 to 150 μ L L-1 for juvenile Colossoma macropomum.
摘要:
这项研究的目的是表征浓度为125μLL-1,150μL-1,175μL-1和200μL-1的NepetaCataria(EON)精油的活性。在浸浴期间,姿势反射丧失,姿势反射和心电图活动恢复的行为,并记录了大型体瘤的手术搏动,持续5分钟,以便在麻醉期间获得安全使用的窗口。鱼(23.38±3.5g)被分配到以下实验中:实验1(姿势反射丢失和恢复的潜伏期):(a)125μLL-1,(b)150μLL-1,(c)175μL-1和(d)200μL-1(n=9)。实验2(心电图和心跳记录):(a)对照组;(b)载体对照组(每升水2毫升酒精),(c)125μLL-1,(d)150μLL-1,(e)175μLL-1和(f)200μLL-1(n=9),每组。所有使用的浓度均显示出诱导姿势反射丧失和姿势反射恢复的可逆性,但是心电图记录显示了形态变化,例如在所测试的最高浓度下,诱导过程中的心动过缓和恢复过程中的p波形成。这样,我们建议使用EON短期麻醉的安全使用窗口,其浓度范围为125至150μL-1。