PDF(Pubmed)
Abstract：
Nutria, or coypu (Myocastor coypus), are invasive semi-aquatic rodents present across the United States, Europe, and Asia. Despite successful eradication efforts in certain areas, nutria have resurged in the mid-Atlantic USA, underscoring the need for advanced monitoring tools. Environmental DNA (eDNA) has emerged as a promising technique for species detection and monitoring. Here, an eDNA assay for nutria using qPCR was field-validated in Virginia, USA, showcasing its potential as a tool for post-eradication monitoring. The findings reveal an association between water levels and detection of nutria eDNA, highlighting the importance of water levels in nutria behavior. A painted turtle assay was introduced to confirm nutria absence and demonstrate the potential of passive sampling. The study showcases the sensitivity and efficiency of eDNA assays, emphasizing their value for monitoring and verifying invasive species eradication.
摘要：
Nutria,或coypu（Myocastorcoypu），是美国各地存在的侵入性半水生啮齿动物，欧洲,和亚洲。尽管在某些地区进行了成功的根除努力，在美国大西洋中部重新流行，强调需要先进的监测工具。环境DNA（eDNA）已成为一种有前途的物种检测和监测技术。这里,使用qPCR对营养进行的eDNA分析在弗吉尼亚州进行了现场验证，美国,展示其作为根除后监测工具的潜力。这些发现揭示了水位和营养eDNA检测之间的联系，强调营养行为中水位的重要性。引入了涂漆的乌龟测定法，以确认无营养，并证明了被动采样的潜力。这项研究展示了eDNA检测的灵敏度和效率，强调其监测和验证入侵物种根除的价值。
