PDF(Pubmed)
Abstract：
UNASSIGNED: Ageing is a key risk factor for cardiovascular disease and is linked to several alterations in cardiac structure and function, including left ventricular hypertrophy and increased cardiomyocyte volume, as well as a decline in the number of cardiomyocytes and ventricular dysfunction, emphasizing the pathological impacts of cardiomyocyte ageing. Dental pulp stem cells (DPSCs) are promising as a cellular therapeutic source due to their minimally invasive surgical approach and remarkable proliferative ability.
UNASSIGNED: This study is the first to investigate the outcomes of the systemic transplantation of DPSCs in a D-galactose (D-gal)-induced rat model of cardiac ageing. Methods. Thirty 9-week-old Sprague-Dawley male rats were randomly assigned into three groups: control, ageing (D-gal), and transplanted groups (D-gal + DPSCs). D-gal (300 mg/kg/day) was administered intraperitoneally daily for 8 weeks. The rats in the transplantation group were intravenously injected with DPSCs at a dose of 1 × 106 once every 2 weeks.
UNASSIGNED: The transplanted cells migrated to the heart, differentiated into cardiomyocytes, improved cardiac function, upregulated Sirt1 expression, exerted antioxidative effects, modulated connexin-43 expression, attenuated cardiac histopathological alterations, and had anti-senescent and anti-apoptotic effects.
UNASSIGNED: Our results reveal the beneficial effects of DPSC transplantation in a cardiac ageing rat model, suggesting their potential as a viable cell therapy for ageing hearts.
UNASSIGNED: This study is the first to investigate the outcomes of the systemic transplantation of DPSCs in a D-galactose (D-gal)-induced rat model of cardiac ageing. Methods. Thirty 9-week-old Sprague-Dawley male rats were randomly assigned into three groups: control, ageing (D-gal), and transplanted groups (D-gal + DPSCs). D-gal (300 mg/kg/day) was administered intraperitoneally daily for 8 weeks. The rats in the transplantation group were intravenously injected with DPSCs at a dose of 1 × 106 once every 2 weeks.
UNASSIGNED: The transplanted cells migrated to the heart, differentiated into cardiomyocytes, improved cardiac function, upregulated Sirt1 expression, exerted antioxidative effects, modulated connexin-43 expression, attenuated cardiac histopathological alterations, and had anti-senescent and anti-apoptotic effects.
UNASSIGNED: Our results reveal the beneficial effects of DPSC transplantation in a cardiac ageing rat model, suggesting their potential as a viable cell therapy for ageing hearts.
摘要：
衰老是心血管疾病的关键危险因素，与心脏结构和功能的若干改变有关。包括左心室肥厚和心肌细胞体积增加，以及心肌细胞数量和心室功能障碍的减少，强调心肌细胞老化的病理影响。牙髓干细胞（DPSC）由于其微创手术方法和显着的增殖能力而有望作为细胞治疗来源。
■本研究首次研究了在D-半乳糖（D-gal）诱导的心脏衰老大鼠模型中DPSC的全身移植结果。方法。将30只9周龄的Sprague-Dawley雄性大鼠随机分为三组：对照组，老化(D-gal)，和移植组(D-gal+DPSC)。每天腹膜内施用D-gal(300mg/kg/天),持续8周。移植组大鼠每2周静脉注射1×106剂量的DPSC。
■移植的细胞迁移到心脏，分化为心肌细胞，改善心脏功能，上调Sirt1表达，发挥抗氧化作用，调节连接蛋白-43表达，减弱的心脏组织病理学改变，并具有抗衰老和抗凋亡作用。
■我们的结果揭示了DPSC移植在心脏老化大鼠模型中的有益效果，表明它们作为衰老心脏的可行细胞疗法的潜力。
■本研究首次研究了在D-半乳糖（D-gal）诱导的心脏衰老大鼠模型中DPSC的全身移植结果。方法。将30只9周龄的Sprague-Dawley雄性大鼠随机分为三组：对照组，老化(D-gal)，和移植组(D-gal+DPSC)。每天腹膜内施用D-gal(300mg/kg/天),持续8周。移植组大鼠每2周静脉注射1×106剂量的DPSC。
■移植的细胞迁移到心脏，分化为心肌细胞，改善心脏功能，上调Sirt1表达，发挥抗氧化作用，调节连接蛋白-43表达，减弱的心脏组织病理学改变，并具有抗衰老和抗凋亡作用。
■我们的结果揭示了DPSC移植在心脏老化大鼠模型中的有益效果，表明它们作为衰老心脏的可行细胞疗法的潜力。
