UNASSIGNED: This study is the first to investigate the outcomes of the systemic transplantation of DPSCs in a D-galactose (D-gal)-induced rat model of cardiac ageing. Methods. Thirty 9-week-old Sprague-Dawley male rats were randomly assigned into three groups: control, ageing (D-gal), and transplanted groups (D-gal + DPSCs). D-gal (300 mg/kg/day) was administered intraperitoneally daily for 8 weeks. The rats in the transplantation group were intravenously injected with DPSCs at a dose of 1 × 106 once every 2 weeks.
UNASSIGNED: The transplanted cells migrated to the heart, differentiated into cardiomyocytes, improved cardiac function, upregulated Sirt1 expression, exerted antioxidative effects, modulated connexin-43 expression, attenuated cardiac histopathological alterations, and had anti-senescent and anti-apoptotic effects.
UNASSIGNED: Our results reveal the beneficial effects of DPSC transplantation in a cardiac ageing rat model, suggesting their potential as a viable cell therapy for ageing hearts.
■本研究首次研究了在D-半乳糖(D-gal)诱导的心脏衰老大鼠模型中DPSC的全身移植结果。方法。将30只9周龄的Sprague-Dawley雄性大鼠随机分为三组:对照组,老化(D-gal),和移植组(D-gal+DPSC)。每天腹膜内施用D-gal(300mg/kg/天),持续8周。移植组大鼠每2周静脉注射1×106剂量的DPSC。
■移植的细胞迁移到心脏,分化为心肌细胞,改善心脏功能,上调Sirt1表达,发挥抗氧化作用,调节连接蛋白-43表达,减弱的心脏组织病理学改变,并具有抗衰老和抗凋亡作用。
■我们的结果揭示了DPSC移植在心脏老化大鼠模型中的有益效果,表明它们作为衰老心脏的可行细胞疗法的潜力。