PDF(Pubmed)
Abstract：
Recent studies have demonstrated that cytokine dysregulation has a critical role in the pathogenesis of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The aim of this study was to investigate the association between tumor necrosis factor (TNF- α), interleukin 6 (IL-6), interleukin 10 (IL-10), and interleukin 17 (IL-17) with infection status, and severity of dengue. A prospective cross-sectional study was conducted at three hospitals in Gianyar regency and Denpasar municipality, Bali, Indonesia, from June to December 2022. Sixty-four dengue infected patients were involved. Patients\' serum was tested for dengue infection using NS1 antigen rapid test, dengue virus immunoglobulin M (IgM) and immunoglobulin G (IgG) test, and reverse transcription polymerase chain reaction (RT-PCR). Cytokine levels (TNF-α, IL-6, IL-10, and IL-17) were measured using enzyme-linked immunosorbent assay (ELISA). Infection status was determined by combining serological and RT-PCR results, categorizing patients into primary and secondary infections. The present study found that DF patients had lower TNF-α, IL-6, and IL-17 but higher IL-10 levels compared to DHF patients (p<0.001). Elevated TNF-α, IL-6, and IL-17 levels were higher in secondary infection, while IL-10 level was higher in primary infection (p<0.001). In conclusion, cytokines play a crucial role in the interplay between cytokine dysregulation and dengue infection dynamics.
摘要：
最近的研究表明，细胞因子失调在登革出血热（DHF）和登革休克综合征（DSS）的发病机理中起着至关重要的作用。本研究的目的是探讨肿瘤坏死因子(TNF-α)和白细胞介素6（IL-6），白细胞介素10（IL-10），和白细胞介素17（IL-17）与感染状态，和登革热的严重程度。在GianyarRegency和Denpasar市的三家医院进行了前瞻性横断面研究，巴厘岛,印度尼西亚,2022年6月至12月。涉及64例登革热感染患者。使用NS1抗原快速检测法对患者血清进行登革热感染检测，登革病毒免疫球蛋白M（IgM）和免疫球蛋白G（IgG）测试，逆转录聚合酶链反应(RT-PCR)。细胞因子水平（TNF-α，使用酶联免疫吸附测定（ELISA）测量IL-6，IL-10和IL-17）。结合血清学和RT-PCR结果确定感染状态，将患者分为原发性和继发性感染。本研究发现DF患者的TNF-α水平较低,IL-6和IL-17，但与DHF患者相比，IL-10水平更高（p<0.001）。TNF-α升高,IL-6和IL-17水平在继发感染中更高，而IL-10水平在原发感染中较高(p<0.001)。总之,细胞因子在细胞因子失调和登革热感染动力学之间的相互作用中起着至关重要的作用。
