Abstract：
Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better visualizing the dynamic behaviors of various subcellular organelles in living cells. By harnessing the versatility of this technique to capture sequential images, we could observe morphological changes in cellular geometry after virus infection in real time without probes or invasive staining. The tune-up APC microscopy system is a highly efficient platform for simultaneously observing the dynamic behaviors of diverse subcellular structures with exceptional resolution.
摘要：
细胞生物学家长期以来一直寻求在没有标记的情况下观察活细胞中的细胞内结构的能力。这项研究提出了调整市售变迹相衬（APC）显微镜系统的程序，以更好地可视化活细胞中各种亚细胞细胞器的动态行为。通过利用这种技术的多功能性来捕获连续图像，我们可以实时观察病毒感染后细胞几何形态的变化，无需探针或侵入性染色。调整APC显微镜系统是一个高效的平台，可同时观察具有出色分辨率的各种亚细胞结构的动态行为。关键词:无标记成像,细胞器动力学,病毒感染,相衬变迹。
