Abstract：
OBJECTIVE: To decipher the role of locally injected bone marrow mesenchymal stem cells (BM-MSCs) in the tongue of hypothyroid rats.
METHODS: A total 24 male Wister rats were utilized and allocated into 3 groups (n = 8). As for the control group, rats received distilled water via oral gavage. In the hypothyroid group, rats administered carbimazole 5 mg/ 250 g/ day for 6 successive weeks, for hypothyroidism induction. The BM-MSC treated hypothyroid group (BM-MSC group); hypothyroid rats received local injection of 0.5 million BM-MSCs in tongue. Six weeks after BM-MSC injection, tongue samples were processed for Hematoxylin and eosin (H and E) staining, Ki67-immunohistochemistry and histomorphometric analysis.
RESULTS: The hypothyroid group revealed degenerative alterations in the lingual papillae, and apparent thinning of the inferior lingual epithelium compared to their controls. Tongues of the BM-MSC group depicted restoration of the normal tongue histology. The Ki67 immunoreaction was apparently decreased in the lingual epithelium of hypothyroid group compared to their controls, however the BM-MSC group regained Ki67 immunostaining.
CONCLUSIONS: Our data suggest that administration of BM-MSCs rescued the degenerative changes in the lingual mucosa and one of the possible underlying mechanisms could be the restoration of cellular proliferation in the lingual epithelium.
METHODS: A total 24 male Wister rats were utilized and allocated into 3 groups (n = 8). As for the control group, rats received distilled water via oral gavage. In the hypothyroid group, rats administered carbimazole 5 mg/ 250 g/ day for 6 successive weeks, for hypothyroidism induction. The BM-MSC treated hypothyroid group (BM-MSC group); hypothyroid rats received local injection of 0.5 million BM-MSCs in tongue. Six weeks after BM-MSC injection, tongue samples were processed for Hematoxylin and eosin (H and E) staining, Ki67-immunohistochemistry and histomorphometric analysis.
RESULTS: The hypothyroid group revealed degenerative alterations in the lingual papillae, and apparent thinning of the inferior lingual epithelium compared to their controls. Tongues of the BM-MSC group depicted restoration of the normal tongue histology. The Ki67 immunoreaction was apparently decreased in the lingual epithelium of hypothyroid group compared to their controls, however the BM-MSC group regained Ki67 immunostaining.
CONCLUSIONS: Our data suggest that administration of BM-MSCs rescued the degenerative changes in the lingual mucosa and one of the possible underlying mechanisms could be the restoration of cellular proliferation in the lingual epithelium.
摘要：
目的：了解局部注射骨髓间充质干细胞(BM-MSCs)在甲状腺功能减退大鼠舌内的作用。
方法:使用总共24只雄性Wister大鼠并将其分成3组(n=8)。至于对照组，大鼠通过口服灌胃接受蒸馏水。甲状腺功能减退组,大鼠服用卡比马唑5毫克/250克/天连续6周，甲状腺功能减退的诱导。BM-MSC治疗甲状腺功能减退组(BM-MSC组);甲状腺功能减退大鼠舌内局部注射50万个BM-MSC。BM-MSC注射后六周，舌样进行苏木精和伊红（H和E）染色处理，Ki67免疫组织化学和组织形态计量学分析。
结果：甲状腺功能减退组显示舌乳头变性改变，与对照组相比，舌下上皮明显变薄。BM-MSC组的舌头描绘了正常舌头组织学的恢复。与对照组相比，甲状腺功能减退组舌上皮的Ki67免疫反应明显减少，然而,BM-MSC组恢复了Ki67免疫染色.
结论：我们的数据表明，给予BM-MSCs挽救了舌粘膜的退行性变化，可能的潜在机制之一可能是舌上皮细胞增殖的恢复。
方法:使用总共24只雄性Wister大鼠并将其分成3组(n=8)。至于对照组，大鼠通过口服灌胃接受蒸馏水。甲状腺功能减退组,大鼠服用卡比马唑5毫克/250克/天连续6周，甲状腺功能减退的诱导。BM-MSC治疗甲状腺功能减退组(BM-MSC组);甲状腺功能减退大鼠舌内局部注射50万个BM-MSC。BM-MSC注射后六周，舌样进行苏木精和伊红（H和E）染色处理，Ki67免疫组织化学和组织形态计量学分析。
结果：甲状腺功能减退组显示舌乳头变性改变，与对照组相比，舌下上皮明显变薄。BM-MSC组的舌头描绘了正常舌头组织学的恢复。与对照组相比，甲状腺功能减退组舌上皮的Ki67免疫反应明显减少，然而,BM-MSC组恢复了Ki67免疫染色.
结论：我们的数据表明，给予BM-MSCs挽救了舌粘膜的退行性变化，可能的潜在机制之一可能是舌上皮细胞增殖的恢复。
