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Abstract：
The Mayaro virus (MAYV) is an arbovirus with emerging potential, though with a limited understanding of its epidemiology and evolution due to the lack of studies and surveillance. Here, we investigated 71 MAYV genome sequences from the Americas available at GenBank and characterized the phylogenetic relationship among virus strains. A phylogenetic analysis showed that sequences were grouped according to the genotypes L, D, and N. Genotype D sequences were closely related to sequences collected in adjacent years and from their respective countries, suggesting that isolates may have originated from circulating lineages. The coalescent analysis demonstrated similar results, indicating the continuous circulation of the virus between countries as well. An unidentified sequence from the USA was grouped with genotype D, suggesting the insertion of this genotype in the country. Furthermore, the recombination analysis detected homologous and three heterologous hybrids which presented an insertion into the nsP3 protein. Amino acid substitutions among sequences indicated selective pressure sites, suggesting viral adaptability. This also impacted the binding affinity between the E1-E2 protein complex and the Mxra8 receptor, associated with MAYV entry into human cells. These results provide information for a better understanding of genotypes circulating in the Americas.
摘要：
Mayaro病毒（MAYV）是一种具有新兴潜力的虫媒病毒，尽管由于缺乏研究和监测，对其流行病学和演变的了解有限。这里,我们调查了GenBank提供的来自美洲的71个MAYV基因组序列，并表征了病毒株之间的系统发育关系。系统发育分析表明，序列根据基因型L进行分组，D,基因型D序列与相邻年份和各自国家收集的序列密切相关，表明分离株可能起源于循环谱系。聚结分析显示出类似的结果，表明病毒在国家之间的持续传播。来自美国的未知序列与基因型D分组，这表明在该国插入了这种基因型。此外，重组分析检测到同源和三个异源杂种，它们在nsP3蛋白中插入。序列中的氨基酸取代表明选择性压力位点，提示病毒适应性。这也影响了E1-E2蛋白复合物和Mxra8受体之间的结合亲和力，与MAYV进入人类细胞有关。这些结果为更好地了解美洲流行的基因型提供了信息。
