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Abstract：
Plague is an endemic infectious disease caused by Yersinia pestis. In this study, we isolated fourteen phages with similar sequence arrangements to phage 186; these phages exhibited different lytic abilities in Enterobacteriaceae strains. To illustrate the phylogenetic relationships and evolutionary relationships between previously designated 186-type phages, we analysed the complete sequences and important genes of the phages, including whole-genome average nucleotide identity (ANI) and collinearity comparison, evolutionary analysis of four conserved structural genes (V, T, R, and Q genes), and analysis of the regulatory genes (cI, apl, and cII) and integrase gene (int). Phylogenetic analysis revealed that thirteen of the newly isolated phages belong to the genus Eganvirus and one belongs to the genus Felsduovirus in the family Peduoviridae, and these Eganvirus phages can be roughly clustered into three subgroups. The topological relationships exhibited by the whole-genome and structural genes seemed similar and stable, while the regulatory genes presented different topological relationships with the structural genes, and these results indicated that there was some homologous recombination in the regulatory genes. These newly isolated 186-type phages were mostly isolated from dogs, suggesting that the resistance of Canidae to Y. pestis infection may be related to the wide distribution of phages with lytic capability.
摘要：
鼠疫是由鼠疫耶尔森氏菌引起的地方性传染病。在这项研究中,我们分离出14个与噬菌体186具有相似序列排列的噬菌体；这些噬菌体在肠杆菌科菌株中表现出不同的裂解能力。为了说明先前指定的186型噬菌体之间的系统发育关系和进化关系，我们分析了噬菌体的完整序列和重要基因，包括全基因组平均核苷酸同一性（ANI）和共线性比较，四个保守结构基因的进化分析(V，T,R,和Q基因)，和调节基因的分析(CI，apl,和cII）和整合酶基因（int）。系统发育分析显示，新分离的噬菌体中有13个属于E角病毒属，一个属于小病毒科中的Felsduovirus属，而这些埃博拉病毒噬菌体可以大致分为三个亚组。全基因组和结构基因表现出的拓扑关系似乎相似和稳定，虽然调控基因与结构基因呈现不同的拓扑关系，这些结果表明调控基因中存在一些同源重组。这些新分离的186型噬菌体大部分是从狗中分离的，提示犬科对鼠疫耶尔森氏菌感染的抗性可能与具有裂解能力的噬菌体的广泛分布有关。
