PDF(Pubmed)
Abstract：
In September 2021, 14 smallmouth bass (SMB; Micropterus dolomieu) with skin lesions were collected from Green Bay waters of Lake Michigan and submitted for diagnostic evaluation. All the skin samples tested positive for largemouth bass virus (LMBV) by conventional PCR. The complete genome of the LMBV (99,328 bp) isolated from a homogenized skin sample was determined using an Illumina MiSeq sequencer. A maximum likelihood (ML) phylogenetic analysis based on the 21 core iridovirus genes supported the LMBV isolated from SMB (LMBV-WVL21117) as a member of the species Santee-Cooper ranavirus. Pairwise nucleotide comparison of the major capsid protein (MCP) gene showed that LMBV-WVL21117 is identical to other LMBV reported from the United States and nearly identical to doctor fish virus and guppy virus 6 (99.2%) from Southeast Asia, as well as LMBV isolates from China and Thailand (99.1%). In addition, ML phylogenetic analysis based on the MCP gene suggests three genotypes of LMBV separated by region: genotype one from the United States, genotype two from Southeast Asia, and genotype three from China and Thailand. Additional research is needed to understand the prevalence and genetic diversity of LMBV strains circulating in wild and managed fish populations from different regions.
摘要：
2021年9月，从密歇根湖的格林贝水域收集了14个皮肤病变的小嘴鲈鱼（SMB；Micropterusdolomieu），并提交诊断评估。所有皮肤样品通过常规PCR检测为大口鲈鱼病毒(LMBV)阳性。使用IlluminaMiSeq测序仪测定从均质化皮肤样品分离的LMBV(99,328bp)的完整基因组。基于21个核心虹膜病毒基因的最大似然（ML）系统发育分析支持从SMB分离的LMBV（LMBV-WVL21117）作为Santee-Cooperranavirus物种的成员。主要衣壳蛋白（MCP）基因的成对核苷酸比较显示，LMBV-WVL21117与美国报道的其他LMBV相同，与东南亚的医生鱼病毒和孔雀鱼病毒6（99.2％）几乎相同，以及来自中国和泰国的LMBV分离株(99.1%)。此外,基于MCP基因的ML系统发育分析表明，LMBV的三种基因型按区域分开：基因型一来自美国，基因型2来自东南亚，基因型3来自中国和泰国。需要更多的研究来了解不同地区野生和管理鱼类种群中LMBV菌株的流行和遗传多样性。
