PDF(Pubmed)
Abstract：
Vibrio species are naturally found in estuarine and marine ecosystems, but are also recognized as significant human enteropathogens, often linked to seafood-related illnesses. In aquaculture settings, Vibrio poses a substantial risk of infectious diseases, resulting in considerable stock losses and prompting the use of antimicrobials. However, this practice contributes to the proliferation of antimicrobial-resistant (AMR) bacteria and resistance genes. Our investigation aimed to explore the potential of biological agents such as bacteriophage CH20 and endolysin LysVPp1 in reducing Vibrio bacterial loads in both rotifer and fish larvae. LysVPp1\'s lytic activity was assessed by measuring absorbance reduction against various pathogenic Vibrio strains. Phage CH20 exhibited a limited host range, affecting only Vibrio alginolyticus GV09, a highly pathogenic strain. Both CH20 and LysVPp1 were evaluated for their effectiveness in reducing Vibrio load in rotifers or fish larvae through short-setting bioassays. Our results demonstrated the significant lytic effect of endolysin LysVPp1 on strains of Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio splendidus. Furthermore, we have showcased the feasibility of reducing the load of pathogenic Vibrio in live feed and fish larvae by using a non-antibiotic-based approach, such as lytic phage and endolysin LysVPp1, thus contributing to the progress of a sustainable aquaculture from a One Health perspective.
摘要：
弧菌属自然存在于河口和海洋生态系统中，但也被认为是重要的人类肠病原体，通常与海鲜相关的疾病有关。在水产养殖环境中，弧菌构成传染病的巨大风险，导致大量的库存损失，并促使抗菌药物的使用。然而,这种做法有助于抗菌素耐药（AMR）细菌和耐药基因的增殖。我们的研究旨在探索噬菌体CH20和内溶素LysVPp1等生物制剂在减少轮虫和鱼幼虫中弧菌细菌负荷方面的潜力。通过测量针对各种致病性弧菌菌株的吸光度降低来评估LysVPp1的裂解活性。噬菌体CH20表现出有限的宿主范围，仅影响溶藻弧菌GV09，一种高致病性菌株。通过短期生物测定法评估了CH20和LysVPp1在减少轮虫或鱼幼虫中弧菌负荷方面的有效性。我们的结果表明，内溶素LysVPp1对溶藻弧菌菌株具有显着的裂解作用，副溶血性弧菌,和脾弧菌.此外，我们已经展示了通过使用非基于抗生素的方法减少活饲料和鱼幼虫中致病性弧菌负荷的可行性，例如裂解噬菌体和内溶素LysVPp1，从而从一个健康的角度为可持续水产养殖的进步做出贡献。
