关键词: bioactive compounds extraction methods high-pressure processing microwave-assisted extraction olive pomace pulsed electric fields

Mesh : Microwaves Olea / chemistry Antioxidants / chemistry isolation & purification Pressure Electricity Plant Extracts / chemistry Solvents / chemistry Olive Oil / chemistry Methanol / chemistry

来  源:   DOI:10.3390/molecules29102303   PDF(Pubmed)

Abstract:
Olive oil production is characterized by large amounts of waste, and yet is considerably highly valued. Olive pomace can serve as a cheap source of bioactive compounds (BACs) with important antioxidant activity. Novel technologies like Pulsed Electric Fields (PEF) and High Pressure (HP) and microwave (MW) processing are considered green alternatives for the recovery of BACs. Different microwave (150-600 W), PEF (1-5 kV/cm field strength, 100-1500 pulses/15 µs width), and HP (250-650 MPa) conditions, in various product/solvent ratios, methanol concentrations, extraction temperatures, and processing times were investigated. Results indicated that the optimal MW extraction conditions were 300 W at 50 °C for 5 min using 60% v/v methanol with a product/solvent ratio of 1:10 g/mL. Similarly, the mix of 40% v/v methanol with olive pomace, treated at 650 MPa for the time needed for pressure build-up (1 min) were considered as optimal extraction conditions in the case of HP, while for PEF the optimal conditions were 60% v/v methanol with a product/solvent ratio of 1:10 g/mL, treated at 5000 pulses, followed by 1 h extraction under stirring conditions. Therefore, these alternative extraction technologies could assist the conventional practice in minimizing waste production and simultaneously align with the requirements of the circular bioeconomy concept.
摘要:
橄榄油生产的特点是大量的废物,然而,它的价值相当高。橄榄渣可以作为具有重要抗氧化活性的生物活性化合物(BACs)的廉价来源。脉冲电场(PEF),高压(HP)和微波(MW)处理等新技术被认为是回收BAC的绿色替代品。不同的微波(150-600W),PEF(1-5kV/cm场强,100-1500脉冲/15µs宽度),和HP(250-650MPa)条件,在各种产品/溶剂比率下,甲醇浓度,提取温度,和处理时间进行了调查。结果表明,最佳的MW提取条件是使用60%v/v甲醇在50°C下300W持续5分钟,产品/溶剂比为1:10g/mL。同样,40%v/v甲醇与橄榄果渣的混合物,在650MPa下处理压力建立所需的时间(1分钟)被认为是HP的最佳提取条件,而对于PEF,最佳条件是60%v/v甲醇,产品/溶剂比为1:10g/mL,在5000个脉冲下治疗,然后在搅拌条件下提取1小时。因此,这些替代提取技术可以帮助减少废物产生的常规做法,同时符合循环生物经济概念的要求。
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