PDF(Pubmed)
Abstract：
Chemokines, also known as chemotactic cytokines, stimulate the migration of immune cells. These molecules play a key role in the pathogenesis of inflammation leading to atherosclerosis, neurodegenerative disorders, rheumatoid arthritis, insulin-resistant diabetes, and cancer. Moreover, they take part in inflammatory bowel disease (IBD). The main objective of our research was to determine the activity of methyl-derivatives of flavanone, namely, 2\'-methylflavanone (5B), 3\'-methylflavanone (6B), 4\'-methylflavanone (7B), and 6-methylflavanone (8B), on the releasing of selected cytokines by RAW264.7 macrophages activated by LPS. We determined the concentration of chemokines belonging to the CC chemokine family, namely, MCP-1, MIP-1β, RANTES, and eotaxin, using the Bio-Plex Magnetic Luminex Assay and the Bio-PlexTM 200 System. Among the tested compounds, only 5B and 6B had the strongest effect on inhibiting the examined chemokines\' release by macrophages. Therefore, 5B and 6B appear to be potentially useful in the prevention of diseases associated with the inflammatory process.
摘要：
趋化因子,也被称为趋化细胞因子，刺激免疫细胞的迁移。这些分子在导致动脉粥样硬化的炎症的发病机理中起关键作用，神经退行性疾病，类风湿性关节炎,胰岛素抵抗糖尿病，和癌症。此外，他们参与炎症性肠病（IBD）。我们研究的主要目的是确定黄烷酮的甲基衍生物的活性，即,2'-甲基黄烷酮（5B），3'-甲基黄烷酮（6B），4'-甲基黄烷酮（7B），和6-甲基黄烷酮（8B），通过LPS激活的RAW264.7巨噬细胞释放选定的细胞因子。我们确定了属于CC趋化因子家族的趋化因子的浓度，即,MCP-1,MIP-1β,RANTES,和eotaxin，使用Bio-Plex磁性Luminex测定和Bio-PlexTM200系统。在测试的化合物中，只有5B和6B对所检查的巨噬细胞释放趋化因子的抑制作用最强。因此,图5B和6B似乎潜在地可用于预防与炎症过程相关的疾病。
