PDF(Pubmed)
Abstract：
Rice (Oryza sativa) is one of the most important crops for humans. The homologs of ent-kaurene synthase (KS) in rice, which are responsible for the biosynthesis of gibberellins and various phytoalexins, are identified by their distinct biochemical functions. However, the KS-Like (KSL) family\'s potential functions related to hormone and abiotic stress in rice remain uncertain. Here, we identified the KSL family of 19 species by domain analysis and grouped 97 KSL family proteins into three categories. Collinearity analysis of KSLs among Poaceae indicated that the KSL gene may independently evolve and OsKSL1 and OsKSL4 likely play a significant role in the evolutionary process. Tissue expression analysis showed that two-thirds of OsKSLs were expressed in various tissues, whereas OsKSL3 and OsKSL5 were specifically expressed in the root and OsKSL4 in the leaf. Based on the fact that OsKSL2 participates in the biosynthesis of gibberellins and promoter analysis, we detected the gene expression profiles of OsKSLs under hormone treatments (GA, PAC, and ABA) and abiotic stresses (darkness and submergence). The qRT-PCR results demonstrated that OsKSL1, OsKSL3, and OsKSL4 responded to all of the treatments, meaning that these three genes can be candidate genes for abiotic stress. Our results provide new insights into the function of the KSL family in rice growth and resistance to abiotic stress.
摘要：
水稻是人类最重要的作物之一。水稻中ent-kauene合酶（KS）的同源物，它们负责赤霉素和各种植物抗毒素的生物合成，通过它们不同的生化功能来识别。然而,KS-like（KSL）家族在水稻中与激素和非生物胁迫相关的潜在功能仍然不确定。这里,我们通过结构域分析鉴定了19种KSL家族,并将97种KSL家族蛋白分为3类.对禾本科植物KSLs的共线性分析表明，KSL基因可能独立进化，OsKSL1和OsKSL4可能在进化过程中起重要作用。组织表达分析表明，三分之二的OsKSLs在各种组织中表达，而OsKSL3和OsKSL5在根中特异性表达，OsKSL4在叶中特异性表达。基于OsKSL2参与赤霉素生物合成的事实和启动子分析,我们检测了激素处理下OsKSLs的基因表达谱(GA，PAC,和ABA）和非生物胁迫（黑暗和淹没）。qRT-PCR结果表明，OsKSL1，OsKSL3和OsKSL4对所有治疗均有反应。这意味着这三个基因可以成为非生物胁迫的候选基因。我们的结果为KSL家族在水稻生长和对非生物胁迫的抗性中的功能提供了新的见解。
