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Abstract：
The coordinated action of VEGF, IGF1/2 and H19 factors influences the development of endometriosis. The aim of this study was to analyze the expression level of these genes in patients with endometriosis. The study group consisted of 100 patients who were diagnosed with endometriosis on laparoscopic and pathological examination. The control group consisted of 100 patients who were found to be free of endometriosis during the surgical procedure and whose eutopic endometrium wasnormal on histopathological examination. These patients were operated on for uterine fibroids. Gene expression was determined by RT-PCR. The expression of the VEGF gene was significantly higher in the samples classified as clinical stage 1-2 compared to the control material (p < 0.05). There was also a statistically significant difference between the samples studied at clinical stages 1-2 and 3-4 (p < 0.01). The expression of the VEGF gene in the group classified as 1-2 was significantly higher. IGF1 gene expression was significantly lower both in the group of samples classified as clinical stages 1-2 and 3-4 compared to the control group (p < 0.05 in both cases). The expression of the H19 gene was significantly lower in the group of samples classified as clinical stage 3-4 compared to the control group (p < 0.01). The reported studies suggest significant roles of VEGF, IGF and H19 expression in the pathogenesis of endometriosis.
摘要：
VEGF的协同作用,IGF1/2和H19因子影响子宫内膜异位症的发生发展。本研究旨在分析这些基因在子宫内膜异位症患者中的表达水平。研究组100例经腹腔镜及病理检查确诊为子宫内膜异位症患者。对照组由100例患者组成，这些患者在手术过程中发现无子宫内膜异位症，其在位子宫内膜在组织病理学检查中正常。这些患者接受了子宫肌瘤手术。通过RT-PCR测定基因表达。与对照材料相比,分类为临床1-2期的样品中VEGF基因的表达显著更高(p<0.05)。在临床阶段1-2和3-4研究的样品之间也存在统计学上的显著差异(p<0.01)。在分类为1-2的组中，VEGF基因的表达明显更高。与对照组相比,在分类为临床阶段1-2和3-4的样品组中IGF1基因表达均显著较低(在两种情况下p<0.05)。H19基因的表达在分类为临床阶段3-4的样品组中显著低于对照组(p<0.01)。报道的研究表明VEGF的重要作用，IGF和H19在子宫内膜异位症发病机制中的表达.
