PDF(Pubmed)
Abstract：
Epidermal melanin synthesis determines an individual\'s skin color. In humans, melanin is formed by melanocytes within the epidermis. The process of melanin synthesis strongly depends on a range of cellular factors, including the fine-tuned interplay with reactive oxygen species (ROS). In this context, a role of cold atmospheric plasma (CAP) on melanin synthesis was proposed due to its tunable ROS generation. Herein, the argon-driven plasma jet kINPen® MED was employed, and its impact on melanin synthesis was evaluated by comparison with known stimulants such as the phosphodiesterase inhibitor IBMX and UV radiation. Different available model systems were employed, and the melanin content of both cultured human melanocytes (in vitro) and full-thickness human skin biopsies (in situ) were analyzed. A histochemical method detected melanin in skin tissue. Cellular melanin was measured by NIR autofluorescence using flow cytometry, and a highly sensitive HPLC-MS method was applied, which enabled the differentiation of eu- and pheomelanin by their degradation products. The melanin content in full-thickness human skin biopsies increased after repeated CAP exposure, while there were only minor effects in cultured melanocytes compared to UV radiation and IBMX treatment. Based on these findings, CAP does not appear to be a useful option for treating skin pigmentation disorders. On the other hand, the risk of hyperpigmentation as an adverse effect of CAP application for wound healing or other dermatological diseases seems to be neglectable.
摘要：
表皮黑色素的合成决定了个体的肤色。在人类中,黑色素由表皮内的黑素细胞形成。黑色素合成的过程很大程度上取决于一系列细胞因子，包括与活性氧（ROS）的微调相互作用。在这种情况下，提出了冷大气等离子体（CAP）对黑色素合成的作用，因为其可调节的ROS生成。在这里，采用氩驱动等离子体射流kINPen®MED，并且通过与已知的刺激物如磷酸二酯酶抑制剂IBMX和UV辐射比较来评估其对黑色素合成的影响。采用了不同的可用模型系统，并分析了培养的人黑素细胞（体外）和全厚度人皮肤活检（原位）的黑色素含量。组织化学方法检测皮肤组织中的黑色素。使用流式细胞术通过NIR自发荧光测量细胞黑色素，并应用了高灵敏度的HPLC-MS方法，这使得它们的降解产物能够区分胡美林和pheomelanin。反复接触CAP后，全厚度人体皮肤活检中的黑色素含量增加，而与UV辐射和IBMX治疗相比，培养的黑素细胞仅有较小的影响。基于这些发现,CAP似乎不是治疗皮肤色素沉着障碍的有用选择。另一方面,色素沉着过度的风险作为CAP应用于伤口愈合或其他皮肤病的不利影响似乎是可以忽略的。
