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Abstract：
Galium genus belongs to the Rubiaceae family, which consists of approximately 14,000 species. In comparison to its well-known relatives, the plastomes of the Galium genus have not been explored so far. The plastomes of this genus have a typical, quadripartite structure, but differ in gene content, since the infA gene is missing in Galium palustre and Galium trfidum. An evaluation of the effectiveness of using entire chloroplast genome sequences as superbarcodes for accurate plant species identification revealed the high potential of this method for molecular delimitation within the genus and tribe. The trnE-UUC-psbD region showed the biggest number of diagnostides (diagnostic nucleotides) which might be new potential barcodes, not only in Galium, but also in other closely related genera. Relative synonymous codon usage (RSCU) appeared to be connected with the phylogeny of the Rubiaceae family, showing that during evolution, plants started preferring specific codons over others.
摘要：
Galium属属于茜草科，由大约14,000种组成。与其知名亲戚相比，到目前为止，尚未探索Galium属的质体。该属的质体具有典型的，四方结构，但是基因含量不同，因为infA基因在Galiumpalustre和Galiumtrifidum中缺失。对使用整个叶绿体基因组序列作为超级条形码进行精确植物物种鉴定的有效性的评估表明，这种方法在属和部落内进行分子划界的潜力很大。trnE-UUC-psbD区域显示出最大数量的诊断（诊断核苷酸），这可能是新的潜在条形码，不仅在Galium中，但也在其他密切相关的属。相对同义密码子使用（RSCU）似乎与茜草科的系统发育有关，表明在进化过程中，植物开始偏爱特定的密码子而不是其他。
