OBJECTIVE: This study aimed to evaluate the synergistic effects of Guaiacum officinale (GO) and Rhodomyrtus Tomentosa (RT) extracts for treating acne vulgaris on sensitive skin by inhibiting inflammation.
METHODS: The phytochemical constituents and antioxidant activity of GO and RT extracts were determined in vitro. The anti-inflammatory effects were investigated in peptidoglycan (PGN)-induced HaCaT cells. Further, a 28-day clinical trial was conducted involving 30 subjects with both sensitive skin and acne to evaluate the efficacy and subjects\' satisfaction.
RESULTS: Total phenolics and flavonoids were detected in GO and RT extracts, the IC50 values for DPPH radical scavenging were 6.15 wt% and 0.76 wt%, respectively. The combination of GO and RT extracts at a 1:1 (v/v) ratio significantly decreased the expression of TLR-2 and TLR-4, as well as the secretion of IL-1α, IL-8, and TNF-α in PGN-induced HaCaT cells, by 2.30-7.93 times compared to GO extract alone (p < 0.05). Moreover, the cream containing 5 wt% the combination significantly improved facial acne and redness (p < 0.05). The number of comedones decreased by 50.00% and papules by 30.65% after 28 days of application. No adverse events were reported and 96.67% of the subjects were satisfied with the treatment.
CONCLUSIONS: The efficacy of the GO and RT extracts in synergistically suppressing inflammation, improving acne vulgaris, and reducing redness. The study offers an effective and non-irritant treatment for acne vulgaris in individuals with sensitive skin.
目的:本研究旨在评估愈创木香(GO)和RhodomyrtusTomentosa(RT)提取物通过抑制炎症来治疗敏感性皮肤寻常痤疮的协同作用。
方法:体外测定GO和RT提取物的植物化学成分和抗氧化活性。在肽聚糖(PGN)诱导的HaCaT细胞中研究了抗炎作用。Further,我们进行了一项为期28天的临床试验,纳入30名同时患有敏感性皮肤和痤疮的受试者,以评估疗效和受试者满意度.
结果:在GO和RT提取物中检测到总酚和类黄酮,DPPH自由基清除的IC50值为6.15wt%和0.76wt%,分别。GO和RT提取物以1:1(v/v)的比例组合显着降低了TLR-2和TLR-4的表达以及IL-1α的分泌,IL-8和TNF-α在PGN诱导的HaCaT细胞中的表达,与单独的GO提取物相比为2.30-7.93倍(p<0.05)。此外,含有5重量%组合的乳膏显著改善面部痤疮和发红(p<0.05)。施用28天后,粉刺数量减少了50.00%,丘疹减少了30.65%。未报告不良事件,96.67%的受试者对治疗感到满意。
结论:GO和RT提取物协同抑制炎症的功效,改善寻常痤疮,减少发红。该研究为敏感皮肤个体的寻常痤疮提供了有效且无刺激性的治疗。