Abstract：
BACKGROUND: Acne vulgaris, a common chronic dermatological condition worldwide, is associated with inflammatory response and Cutibacterium acnes. Individuals with acne vulgaris and sensitive skin have limited suitable treatments due to the skin irritation and side effects exhibited by current hydroxy acidic medications.
OBJECTIVE: This study aimed to evaluate the synergistic effects of Guaiacum officinale (GO) and Rhodomyrtus Tomentosa (RT) extracts for treating acne vulgaris on sensitive skin by inhibiting inflammation.
METHODS: The phytochemical constituents and antioxidant activity of GO and RT extracts were determined in vitro. The anti-inflammatory effects were investigated in peptidoglycan (PGN)-induced HaCaT cells. Further, a 28-day clinical trial was conducted involving 30 subjects with both sensitive skin and acne to evaluate the efficacy and subjects\' satisfaction.
RESULTS: Total phenolics and flavonoids were detected in GO and RT extracts, the IC50 values for DPPH radical scavenging were 6.15 wt% and 0.76 wt%, respectively. The combination of GO and RT extracts at a 1:1 (v/v) ratio significantly decreased the expression of TLR-2 and TLR-4, as well as the secretion of IL-1α, IL-8, and TNF-α in PGN-induced HaCaT cells, by 2.30-7.93 times compared to GO extract alone (p < 0.05). Moreover, the cream containing 5 wt% the combination significantly improved facial acne and redness (p < 0.05). The number of comedones decreased by 50.00% and papules by 30.65% after 28 days of application. No adverse events were reported and 96.67% of the subjects were satisfied with the treatment.
CONCLUSIONS: The efficacy of the GO and RT extracts in synergistically suppressing inflammation, improving acne vulgaris, and reducing redness. The study offers an effective and non-irritant treatment for acne vulgaris in individuals with sensitive skin.
OBJECTIVE: This study aimed to evaluate the synergistic effects of Guaiacum officinale (GO) and Rhodomyrtus Tomentosa (RT) extracts for treating acne vulgaris on sensitive skin by inhibiting inflammation.
METHODS: The phytochemical constituents and antioxidant activity of GO and RT extracts were determined in vitro. The anti-inflammatory effects were investigated in peptidoglycan (PGN)-induced HaCaT cells. Further, a 28-day clinical trial was conducted involving 30 subjects with both sensitive skin and acne to evaluate the efficacy and subjects\' satisfaction.
RESULTS: Total phenolics and flavonoids were detected in GO and RT extracts, the IC50 values for DPPH radical scavenging were 6.15 wt% and 0.76 wt%, respectively. The combination of GO and RT extracts at a 1:1 (v/v) ratio significantly decreased the expression of TLR-2 and TLR-4, as well as the secretion of IL-1α, IL-8, and TNF-α in PGN-induced HaCaT cells, by 2.30-7.93 times compared to GO extract alone (p < 0.05). Moreover, the cream containing 5 wt% the combination significantly improved facial acne and redness (p < 0.05). The number of comedones decreased by 50.00% and papules by 30.65% after 28 days of application. No adverse events were reported and 96.67% of the subjects were satisfied with the treatment.
CONCLUSIONS: The efficacy of the GO and RT extracts in synergistically suppressing inflammation, improving acne vulgaris, and reducing redness. The study offers an effective and non-irritant treatment for acne vulgaris in individuals with sensitive skin.
摘要：
背景：寻常痤疮，一种全球常见的慢性皮肤病，与炎症反应和痤疮皮肤杆菌有关。由于当前羟基酸性药物所表现出的皮肤刺激和副作用,患有寻常痤疮和敏感皮肤的个体具有有限的合适治疗。
目的：本研究旨在评估愈创木香（GO）和RhodomyrtusTomentosa（RT）提取物通过抑制炎症来治疗敏感性皮肤寻常痤疮的协同作用。
方法：体外测定GO和RT提取物的植物化学成分和抗氧化活性。在肽聚糖(PGN)诱导的HaCaT细胞中研究了抗炎作用。Further,我们进行了一项为期28天的临床试验,纳入30名同时患有敏感性皮肤和痤疮的受试者,以评估疗效和受试者满意度.
结果：在GO和RT提取物中检测到总酚和类黄酮，DPPH自由基清除的IC50值为6.15wt%和0.76wt%，分别。GO和RT提取物以1:1(v/v)的比例组合显着降低了TLR-2和TLR-4的表达以及IL-1α的分泌，IL-8和TNF-α在PGN诱导的HaCaT细胞中的表达,与单独的GO提取物相比为2.30-7.93倍(p<0.05)。此外，含有5重量%组合的乳膏显著改善面部痤疮和发红(p<0.05)。施用28天后，粉刺数量减少了50.00%，丘疹减少了30.65%。未报告不良事件，96.67％的受试者对治疗感到满意。
结论：GO和RT提取物协同抑制炎症的功效，改善寻常痤疮，减少发红。该研究为敏感皮肤个体的寻常痤疮提供了有效且无刺激性的治疗。
目的：本研究旨在评估愈创木香（GO）和RhodomyrtusTomentosa（RT）提取物通过抑制炎症来治疗敏感性皮肤寻常痤疮的协同作用。
方法：体外测定GO和RT提取物的植物化学成分和抗氧化活性。在肽聚糖(PGN)诱导的HaCaT细胞中研究了抗炎作用。Further,我们进行了一项为期28天的临床试验,纳入30名同时患有敏感性皮肤和痤疮的受试者,以评估疗效和受试者满意度.
结果：在GO和RT提取物中检测到总酚和类黄酮，DPPH自由基清除的IC50值为6.15wt%和0.76wt%，分别。GO和RT提取物以1:1(v/v)的比例组合显着降低了TLR-2和TLR-4的表达以及IL-1α的分泌，IL-8和TNF-α在PGN诱导的HaCaT细胞中的表达,与单独的GO提取物相比为2.30-7.93倍(p<0.05)。此外，含有5重量%组合的乳膏显著改善面部痤疮和发红(p<0.05)。施用28天后，粉刺数量减少了50.00%，丘疹减少了30.65%。未报告不良事件，96.67％的受试者对治疗感到满意。
结论：GO和RT提取物协同抑制炎症的功效，改善寻常痤疮，减少发红。该研究为敏感皮肤个体的寻常痤疮提供了有效且无刺激性的治疗。
