PDF(Pubmed)
Abstract：
Nucleotide-binding site (NBS) domain genes are one of the superfamily of resistance genes involved in plant responses to pathogens. The current study identified 12,820 NBS-domain-containing genes across 34 species covering from mosses to monocots and dicots. These identified genes are classified into 168 classes with several novel domain architecture patterns encompassing significant diversity among plant species. Several classical (NBS, NBS-LRR, TIR-NBS, TIR-NBS-LRR, etc.) and species-specific structural patterns (TIR-NBS-TIR-Cupin_1-Cupin_1, TIR-NBS-Prenyltransf, Sugar_tr-NBS etc.) were discovered. We observed 603 orthogroups (OGs) with some core (most common orthogroups; OG0, OG1, OG2, etc.) and unique (highly specific to species; OG80, OG82, etc.) OGs with tandem duplications. The expression profiling presented the putative upregulation of OG2, OG6, and OG15 in different tissues under various biotic and abiotic stresses in susceptible and tolerant plants to cotton leaf curl disease (CLCuD). The genetic variation between susceptible (Coker 312) and tolerant (Mac7) Gossypium hirsutum accessions identified several unique variants in NBS genes of Mac7 (6583 variants) and Coker312 (5173 variants). The protein-ligand and proteins-protein interaction showed a strong interaction of some putative NBS proteins with ADP/ATP and different core proteins of the cotton leaf curl disease virus. The silencing of GaNBS (OG2) in resistant cotton through virus-induced gene silencing (VIGS) demonstrated its putative role in virus tittering. The presented study will be further helpful in understanding the plant adaptation mechanism.
摘要：
核苷酸结合位点（NBS）域基因是与植物对病原体的反应有关的抗性基因的超家族之一。目前的研究确定了从苔藓到单子叶植物和双子叶植物的34个物种中的12,820个含有NBS结构域的基因。这些鉴定的基因被分为168类，具有几种新的结构域结构模式，涵盖了植物物种之间的显着多样性。几个经典(NBS，NBS-LRR,TIR-NBS,TIR-NBS-LRR,等。)和物种特异性结构模式(TIR-NBS-TIR-Cupin_1-Cupin_1，TIR-NBS-Prenyltransf，Sugar_tr-NBS等。）被发现了。我们观察到603个正交群（OG）具有一些核心（最常见的正交群；OG0，OG1，OG2等。)和独特(对物种高度特异性；OG80、OG82等。)具有串联重复的OG。表达谱显示，在棉花卷叶病（CLCuD）的易感和耐性植物中，在各种生物和非生物胁迫下，OG2，OG6和OG15在不同组织中的推定上调。易感（Coker312）和耐受性（Mac7）陆地棉之间的遗传变异鉴定了Mac7（6583变体）和Coker312（5173变体）的NBS基因中的几种独特变体。蛋白质-配体和蛋白质-蛋白质相互作用显示了一些推定的NBS蛋白与ADP/ATP和棉花曲叶病病毒的不同核心蛋白的强相互作用。通过病毒诱导的基因沉默（VIGS）在抗性棉花中沉默GaNBS（OG2）证明了其在病毒滴定中的作用。本研究将有助于进一步理解植物适应机制。
