Abstract:
In Foot-and-mouth disease (FMD) enzootic countries, periodic vaccination is the key tool in controlling the disease incidence. Active seromonitoring of the vaccinated population is critical to assess the impact of vaccination. Virus neutralization test (VNT) and enzyme-linked immunosorbent assays (ELISA) are commonly used for antibody detection. Assays like liquid phase blocking ELISA (LPBE) or solid phase competition ELISA (SPCE) are preferred as they do not require handling of live FMDV and are routinely used for seromonitoring or for vaccine potency testing; however, false positives are high in LPBE. Here we report, a monoclonal antibody (mAb) based SPCE as a potential alternate assay for antibody titration. From a panel of 12 mAbs against FMDV serotype A, two mAbs were chosen for the development of SPCE. Based on a set of 453 sera, it was demonstrated that mAb 2C4G11, mAb 6E8D11and polyclonal antibody (pAb) based SPCE had a relative sensitivity of 86.1, 86.1 and 80.3 %; and specificity of 99.6, 99.1 and 99.1 %, respectively. The correlation, repeatability, and level of agreement of the assays were high demonstrating the potential use of mAb in large scale surveillance studies and regular vaccine potency testing.
摘要:
在口蹄疫(FMD)植物性疾病国家,定期接种疫苗是控制疾病发病率的关键工具。疫苗接种人群的积极血清监测对于评估疫苗接种的影响至关重要。病毒中和试验(VNT)和酶联免疫吸附试验(ELISA)通常用于抗体检测。优选液相阻断ELISA(LPBE)或固相竞争ELISA(SPCE)等分析方法,因为它们不需要处理FMDV,并且通常用于血清监测或疫苗效力测试;然而,LPBE的假阳性较高。在这里我们报告,基于单克隆抗体(mAb)的SPCE作为抗体滴定的潜在替代测定法。从一组针对FMDV血清型A的12个mAb中,选择两种单克隆抗体用于SPCE的发展。基于一组453份血清,证明了mAb2C4G11,mAb6E8D11和基于pAb的SPCE的相对灵敏度为86.1、86.1和80.3%;特异性为99.6、99.1和99.1%,分别。相关性,重复性,上述试验的一致性水平很高,证明了mAb在大规模监测研究和常规疫苗效力测试中的潜在用途。