Abstract：
Ovarian development in animals is a complicated biological process, requiring the simultaneous coordination among various genes and pathways. To understand the dynamic changes and molecular regulatory mechanisms of ovarian development in mud crab (Scylla paramamosain), both histological observation and whole transcriptome sequencing of ovarian tissues at different mating stages were implemented in this study. The histological results revealed that ovarian development was delayed in unmated females (60 days after courtship behavior but not mating), who exhibited an oocyte diameter of 56.38 ± 15.17 μm. Conversely, mated females exhibited accelerated the ovarian maturation process, with females reaching ovarian stage III (proliferative stage) 23 days after mating and attained an average oocyte diameter of 132.19 ± 15.07 μm. Thus, mating process is essential in promoting the rapid ovarian development in mud crab. Based on the whole transcriptome sequencing analysis, a total of 518 mRNAs, 1502 lncRNAs, 18 circRNAs and 151 miRNAs were identified to be differentially expressed between ovarian tissues at different mating stages. Notably, six differentially expressed genes (DEGs) associated with ovarian development were identified, including ovary development-related protein, red pigment concentrating hormone receptor, G2/mitotic-specific cyclin-B3-like, lutropin-chorio gonadotropic hormone receptor, renin receptor, and SoxB2. More importantly, both DEGs and targets of differentially expressed non-coding RNAs (DEncRNAs) were enriched in renin-angiotensin system, TGF-β signaling, cell adhesion molecules, MAPK signaling pathway, and ECM-receptor interaction, suggesting that these pathways may play significant roles in the ovarian development of mud crabs. Moreover, competition endogenous RNA (ceRNA) networks were constructed while mRNAs were differentially expressed between mating stages were involved in Gene Ontology (GO) biological processes such as developmental process, reproduction, and growth. These findings could provide solid foundations for the future development of female mud crab maturation enhancement strategy, and improve the understanding of the ovarian maturation process in crustaceans.
摘要：
动物卵巢发育是一个复杂的生物学过程,需要各种基因和途径之间的同时协调。目的了解泥蟹卵巢发育的动态变化及分子调控机制,本研究对不同交配阶段的卵巢组织进行了组织学观察和全转录组测序。组织学结果表明，未交配的雌性卵巢发育延迟（求偶行为后60天，但未交配），卵母细胞直径为56.38±15.17μm。相反，交配的雌性表现出加速的卵巢成熟过程，雌性在交配后23天达到卵巢III期（增殖期），平均卵母细胞直径为132.19±15.07μm。因此，交配过程是促进泥蟹卵巢快速发育的关键。基于全转录组测序分析,总共518个mRNA，1502lncRNAs，18个circRNAs和151个miRNAs被鉴定为在不同交配阶段的卵巢组织之间差异表达。值得注意的是,确定了六个与卵巢发育相关的差异表达基因（DEGs），包括卵巢发育相关蛋白，红色素浓缩激素受体，G2/有丝分裂特异性细胞周期蛋白-B3样，促性腺激素-绒毛膜促性腺激素受体，肾素受体,SoxB2更重要的是,DEGs和差异表达的非编码RNA（DEncRNAs）的靶标在肾素-血管紧张素系统中富集，TGF-β信号,细胞粘附分子，MAPK信号通路,和ECM-受体相互作用，提示这些途径可能在泥蟹卵巢发育中起重要作用。此外，竞争内源性RNA(ceRNA)网络的构建，而mRNAs在交配阶段差异表达，参与基因本体论(GO)生物过程，如发育过程，繁殖，和增长。这些发现可以为未来雌性泥蟹成熟增强策略的发展提供坚实的基础。提高对甲壳类动物卵巢成熟过程的认识。
