PDF(Pubmed)
Abstract：
This study aimed at determining the identity of freshwater snails collected from selected water habitats frequented by wildlife as source of drinking water in the Matebeleland region of Zimbabwe and further screening the identified snails for natural infections with amphistomes using PCR. A total of 487 freshwater snails were collected from six areas in the Matebeleland region of Zimbabwe for identification and screening of amphistome infection. Eight freshwater snail species were morphologically identified and Biomphalaria pfeifferi, Bul. tropicus, Bul. truncatus, Bul. globosus, and L. (R.) natalensis were confirmed using the COI gene. Bulinus tropicus and Phy. acuta were the most abundant species at 33.9% (165/487) and 31.2% (155/487), respectively. DNA of amphistome was detected in 11.9% (58/487) of the collected snails. The highest infection rate was detected in Bul. globosus (44.4%). West Nicholson recorded the highest infection rate (33.9%), and infection was not detected in L. (R.) natalensis, Phy. acuta, and Bellamya spp. Amphistome DNA from M. tuberculata was successfully sequenced and identified as Calicophoron microbothrium. An additional band was detected in M. tuberculata, Bul. tropicus, and Bul. trancatus, which showed a 96.42% similarity to Paragonimus sp. sequence in the GenBank.
摘要：
这项研究旨在确定从津巴布韦Matebeleland地区野生动植物经常光顾的选定水栖息地收集的淡水蜗牛的身份，并使用PCR进一步筛选已鉴定的蜗牛是否受到两栖动物的自然感染。从津巴布韦Matebeleland地区的六个地区共收集了487只淡水蜗牛，用于鉴定和筛查amphistome感染。对8种淡水蜗牛进行了形态学鉴定，并对其进行了鉴定。布尔.热带,布尔.truncatus,布尔.球状,和L.(R.)natalensis用COI基因证实。热带鳞茎和Phy。acuta是最丰富的物种，分别为33.9％（165/487）和31.2％（155/487），分别。在收集的蜗牛中，有11.9％（58/487）检测到了安瓿的DNA。在Bul中检测到最高的感染率。球(44.4%)。西尼科尔森的感染率最高(33.9%),并且在L.中未检测到感染(R.)纳塔尔斯，Phy.阿库塔,还有Bellamyaspp.成功测序了来自结核分枝杆菌的AmphistomeDNA，并鉴定为Calicophoronmicrobothrium。在结核杆菌中检测到额外的条带，布尔.热带,还有Bul.trancatus,与并殖吸虫有96.42%的相似性。在GenBank中的序列。
