PDF(Pubmed)
Abstract：
Sexually transmitted diseases (STDs) are a global concern because approximately 1 million new cases emerge daily. Most STDs are curable, but if left untreated, they can cause severe long-term health implications, including infertility and even death. Therefore, a test enabling rapid and accurate screening and genotyping of STD pathogens is highly awaited. Herein, we present the development of the DNA-based 6STD Genotyping 9G Membrane test, a lateral flow strip membrane assay, for the detection and genotyping of six STD pathogens, including Trichomonas vaginalis, Ureaplasma urealyticum, Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, and Mycoplasma genitalium. Here, we developed a multiplex PCR primer set that allows PCR amplification of genomic materials for these six STD pathogens. We also developed the six ssDNA probes that allow highly efficient detection of the six STD pathogens. The 6STD Genotyping 9G Membrane test lets us obtain the final detection and genotyping results in less than 30 m after PCR at 25 °C. The accuracy of the 6STD Genotyping 9G membrane test in STD genotyping was confirmed by its 100% concordance with the sequencing results of 120 clinical samples. Therefore, the 6STD Genotyping 9G Membrane test emerges as a promising diagnostic tool for precise STD genotyping, facilitating informed decision-making in clinical practice.
摘要：
性传播疾病（STD）是全球关注的问题，因为每天大约有100万新病例出现。大多数性病是可以治愈的，但如果不及时治疗,它们会对健康造成严重的长期影响,包括不孕甚至死亡.因此,人们非常期待一种能够快速准确筛查和基因分型性病病原体的检测方法.在这里，我们介绍了基于DNA的6STD基因分型9G膜测试的发展，横向流条膜测定，用于六种性病病原体的检测和基因分型，包括阴道毛滴虫,解脲脲原体，淋病奈瑟菌,沙眼衣原体,人型支原体,和生殖支原体.这里,我们开发了多重PCR引物组,可以对这6种性病病原体的基因组材料进行PCR扩增.我们还开发了六种ssDNA探针，可以高效检测六种STD病原体。6STD基因分型9G膜测试让我们在25°C的PCR后不到30m的时间内获得最终的检测和基因分型结果。6STD基因分型9G膜测试在STD基因分型中的准确性通过其与120个临床样品的测序结果的100%一致性得到证实。因此,6STD基因分型9G膜测试成为一种有前途的诊断工具，用于精确的STD基因分型，促进临床实践中的知情决策。
