Abstract：
BACKGROUND: Detection of elements in individual cells by inductively coupled plasma (ICP) spectrometry has recently attracted significant interest in biological research, due to the unique ability of ICP spectrometry for trace element analysis. However, performing single-cell analysis using ICP optical emission spectrometry (ICP-OES) remains a challenge due to the small size and discrete nature of cells. This is while ICP-OES can serve as a cost-effective and label-free method for this purpose. Therefore, it is necessary to improve the current ICP-OES technique to facilitate the detection of elements in single cells, thereby unlocking novel applications.
RESULTS: A new conical ICP torch, which has been illustrated to offer better analytical performance than the conventional ones, was applied to achieve the detection of calcium in single micro-sized cells. A new heated chamber was designed and coupled with a high-efficiency nebulizer as the sample introduction system. For the detection of single SiO2 particles, the number of particle events obtained by the new sample introduction system was found to be up to 9 times higher than that of the conventional system without sacrificing the signal intensity. Subsequently, calcium in human breast cancer cells (MDA-MB-231), mice breast cancer cells (Py8119), and mice osteocytes (MLO-Y4) was successfully detected using the new ICP-OES system. The cell detection efficiency turned out to be around 2%-3% which is much higher than that the reported values in previous single-cell ICP-OES research. Finally, as a new application, the effect of Yoda1, a recently identified activator of Piezo1 calcium channel, on osteocytes was investigated. The calcium content in Yoda1-treated MLO-Y4 cells was seen increase by 36% compared to the control sample.
CONCLUSIONS: This research reveals the capability of ICP-OES in single-cell analysis for micro-sized cells which was made possible by the new conical ICP torch and the new sample introduction system. The ability to detect calcium in single mammalian cells enables the first ever application of this technique to assess the impact of the Yoda1 activator on the calcium level in osteocytes.
RESULTS: A new conical ICP torch, which has been illustrated to offer better analytical performance than the conventional ones, was applied to achieve the detection of calcium in single micro-sized cells. A new heated chamber was designed and coupled with a high-efficiency nebulizer as the sample introduction system. For the detection of single SiO2 particles, the number of particle events obtained by the new sample introduction system was found to be up to 9 times higher than that of the conventional system without sacrificing the signal intensity. Subsequently, calcium in human breast cancer cells (MDA-MB-231), mice breast cancer cells (Py8119), and mice osteocytes (MLO-Y4) was successfully detected using the new ICP-OES system. The cell detection efficiency turned out to be around 2%-3% which is much higher than that the reported values in previous single-cell ICP-OES research. Finally, as a new application, the effect of Yoda1, a recently identified activator of Piezo1 calcium channel, on osteocytes was investigated. The calcium content in Yoda1-treated MLO-Y4 cells was seen increase by 36% compared to the control sample.
CONCLUSIONS: This research reveals the capability of ICP-OES in single-cell analysis for micro-sized cells which was made possible by the new conical ICP torch and the new sample introduction system. The ability to detect calcium in single mammalian cells enables the first ever application of this technique to assess the impact of the Yoda1 activator on the calcium level in osteocytes.
摘要：
背景：通过电感耦合等离子体（ICP）光谱法检测单个细胞中的元素最近引起了生物学研究的极大兴趣，由于ICP光谱法对微量元素分析的独特能力。然而,使用ICP光学发射光谱法(ICP-OES)进行单细胞分析仍然是一个挑战,因为细胞的小尺寸和离散性质.这同时ICP-OES可以作为用于此目的的成本有效且无标签的方法。因此,有必要改进目前的ICP-OES技术，以方便单细胞中元素的检测，从而解锁新的应用程序。
结果：一种新型锥形ICP炬，这已经被证明提供了比传统的更好的分析性能，用于实现单个微细胞中钙的检测。设计了一个新的加热室，并与高效雾化器耦合作为样品引入系统。对于单个SiO2颗粒的检测，在不牺牲信号强度的情况下，发现新样品引入系统获得的颗粒事件数量比传统系统高9倍。随后，人乳腺癌细胞中的钙（MDA-MB-231），小鼠乳腺癌细胞（Py8119），使用新的ICP-OES系统成功检测了小鼠骨细胞（MLO-Y4）。细胞检测效率约为2％-3％，远高于先前单细胞ICP-OES研究中报道的值。最后,作为一个新的应用程序，最近发现的Piezo1钙通道激活剂Yoda1的作用，对骨细胞进行了研究。与对照样品相比,观察到Yoda1处理的MLO-Y4细胞中的钙含量增加了36%。
结论：这项研究揭示了ICP-OES在微小细胞的单细胞分析中的能力，这通过新的锥形ICP炬和新的样品引入系统成为可能。在单个哺乳动物细胞中检测钙的能力使得该技术的首次应用能够评估Yoda1激活剂对骨细胞中钙水平的影响。
结果：一种新型锥形ICP炬，这已经被证明提供了比传统的更好的分析性能，用于实现单个微细胞中钙的检测。设计了一个新的加热室，并与高效雾化器耦合作为样品引入系统。对于单个SiO2颗粒的检测，在不牺牲信号强度的情况下，发现新样品引入系统获得的颗粒事件数量比传统系统高9倍。随后，人乳腺癌细胞中的钙（MDA-MB-231），小鼠乳腺癌细胞（Py8119），使用新的ICP-OES系统成功检测了小鼠骨细胞（MLO-Y4）。细胞检测效率约为2％-3％，远高于先前单细胞ICP-OES研究中报道的值。最后,作为一个新的应用程序，最近发现的Piezo1钙通道激活剂Yoda1的作用，对骨细胞进行了研究。与对照样品相比,观察到Yoda1处理的MLO-Y4细胞中的钙含量增加了36%。
结论：这项研究揭示了ICP-OES在微小细胞的单细胞分析中的能力，这通过新的锥形ICP炬和新的样品引入系统成为可能。在单个哺乳动物细胞中检测钙的能力使得该技术的首次应用能够评估Yoda1激活剂对骨细胞中钙水平的影响。
