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Abstract：
BACKGROUND: The continuous progress in nanotechnology is rapid and extensive with overwhelming futuristic aspects. Through modernizing inventive synthesis protocols, a paradigm leapfrogging in novelties and findings are channeled toward fostering human health and sustaining the surrounding environment. Owing to the overpricing and jeopardy of physicochemical synthesizing approaches, the quest for ecologically adequate schemes is incontestable. By developing environmentally friendly strategies, mycosynthesis of nanocomposites has been alluring.
RESULTS: Herein, a novel architecture of binary CuO and TiO2 in nanocomposites form was fabricated using bionanofactory Candida sp., for the first time. For accentuating the structural properties of CuTi nanocomposites (CuTiNCs), various characterization techniques were employed. UV-Vis spectroscopy detected SPR at 350 nm, and XRD ascertained the crystalline nature of a hybrid system. However, absorption peaks at 8, 4.5, and 0.5 keV confirmed the presence of Cu, Ti and oxygen, respectively, in an undefined assemblage of polygonal-spheres of 15-75 nm aggregated in the fungal matrix of biomolecules as revealed by EDX, SEM and TEM. However, FTIR, ζ-potential and TGA reflected long-term stability (- 27.7 mV) of self-functionalized CuTiNCs. Interestingly, a considerable and significant biocide performance was detected at 50 µg/mL of CuTiNCs against some human and plant pathogens, compared to monometallic counterparts. Further, CuTiNCs (200 µg/mL) ceased significantly the development of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans biofilms by 80.3 ± 1.4, 68.7 ± 3.0 and 55.7 ± 3.0%, respectively. Whereas, 64.63 ± 3.5 and 89.82 ± 4.3% antimicrofouling potentiality was recorded for 100 and 200 µg/ml of CuTiNCs, respectively; highlighting their destructive effect against marine microfoulers cells and decaying of their extracellular polymeric skeleton as visualized by SEM. Moreover, CuTiNCs (100 and 200 µg/ml) exerted significantly outstanding disinfection potency within 2 h by reducing the microbial load (i.e., total plate count, mold & yeast, total coliforms and faecal Streptococcus) in domestic and agricultural effluents reached >50%.
CONCLUSIONS: The synergistic efficiency provided by CuNPs and TiNPs in mycofunctionalized CuTiNCs boosted its recruitment as antiphytopathogenic, antibiofilm, antimicrofouling and disinfectant agent in various realms.
RESULTS: Herein, a novel architecture of binary CuO and TiO2 in nanocomposites form was fabricated using bionanofactory Candida sp., for the first time. For accentuating the structural properties of CuTi nanocomposites (CuTiNCs), various characterization techniques were employed. UV-Vis spectroscopy detected SPR at 350 nm, and XRD ascertained the crystalline nature of a hybrid system. However, absorption peaks at 8, 4.5, and 0.5 keV confirmed the presence of Cu, Ti and oxygen, respectively, in an undefined assemblage of polygonal-spheres of 15-75 nm aggregated in the fungal matrix of biomolecules as revealed by EDX, SEM and TEM. However, FTIR, ζ-potential and TGA reflected long-term stability (- 27.7 mV) of self-functionalized CuTiNCs. Interestingly, a considerable and significant biocide performance was detected at 50 µg/mL of CuTiNCs against some human and plant pathogens, compared to monometallic counterparts. Further, CuTiNCs (200 µg/mL) ceased significantly the development of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans biofilms by 80.3 ± 1.4, 68.7 ± 3.0 and 55.7 ± 3.0%, respectively. Whereas, 64.63 ± 3.5 and 89.82 ± 4.3% antimicrofouling potentiality was recorded for 100 and 200 µg/ml of CuTiNCs, respectively; highlighting their destructive effect against marine microfoulers cells and decaying of their extracellular polymeric skeleton as visualized by SEM. Moreover, CuTiNCs (100 and 200 µg/ml) exerted significantly outstanding disinfection potency within 2 h by reducing the microbial load (i.e., total plate count, mold & yeast, total coliforms and faecal Streptococcus) in domestic and agricultural effluents reached >50%.
CONCLUSIONS: The synergistic efficiency provided by CuNPs and TiNPs in mycofunctionalized CuTiNCs boosted its recruitment as antiphytopathogenic, antibiofilm, antimicrofouling and disinfectant agent in various realms.
摘要：
背景：纳米技术的不断进步是迅速而广泛的，具有压倒性的未来方面。通过现代化创新合成方案，创新和发现的范式跨越被引导到促进人类健康和维持周围环境。由于物理化学合成方法的定价过高和危险，寻求生态充足的计划是无可争辩的。通过制定环保战略，纳米复合材料的真菌合成一直很诱人。
结果：这里，使用BionanofactoryCandidasp制造了纳米复合材料形式的二元CuO和TiO2的新型结构。,第一次。为了强调CuTi纳米复合材料(CuTiNC)的结构性能，采用了各种表征技术。紫外-可见光谱在350nm处检测到SPR，和XRD确定了杂化体系的结晶性质。然而,8、4.5和0.5keV处的吸收峰证实了Cu的存在，钛和氧，分别,在EDX揭示的生物分子真菌基质中聚集的15-75nm多边形球体的未定义组合中，SEM和TEM。然而,FTIR,ζ电位和TGA反映了自功能化CuTiNC的长期稳定性(-27.7mV)。有趣的是,在50µg/mL的CuTiNCs对某些人类和植物病原体的作用下，检测到相当大的杀生物剂性能，与单金属同行相比。Further,CuTiNCs（200µg/mL）显著停止金黄色葡萄球菌的发展，铜绿假单胞菌和白色念珠菌生物膜分别为80.3±1.4、68.7±3.0和55.7±3.0％，分别。然而,对于100和200µg/ml的CuTiNCs，记录了64.63±3.5和89.82±4.3％的抗微生物污染潜力，分别；突出了它们对海洋微霉菌细胞的破坏作用，并通过SEM观察了它们的细胞外聚合物骨架的衰变。此外，CuTiNCs（100和200µg/ml）在2小时内通过减少微生物负荷（即，总平板计数，霉菌和酵母，家庭和农业废水中的总大肠杆菌和粪便链球菌）达到>50%。
结论：CuNPs和TiNPs在真菌功能化的CuTiNCs中提供的协同效率增强了其作为抗植物病原性的募集，抗生物膜,不同领域的防污剂和消毒剂。
结果：这里，使用BionanofactoryCandidasp制造了纳米复合材料形式的二元CuO和TiO2的新型结构。,第一次。为了强调CuTi纳米复合材料(CuTiNC)的结构性能，采用了各种表征技术。紫外-可见光谱在350nm处检测到SPR，和XRD确定了杂化体系的结晶性质。然而,8、4.5和0.5keV处的吸收峰证实了Cu的存在，钛和氧，分别,在EDX揭示的生物分子真菌基质中聚集的15-75nm多边形球体的未定义组合中，SEM和TEM。然而,FTIR,ζ电位和TGA反映了自功能化CuTiNC的长期稳定性(-27.7mV)。有趣的是,在50µg/mL的CuTiNCs对某些人类和植物病原体的作用下，检测到相当大的杀生物剂性能，与单金属同行相比。Further,CuTiNCs（200µg/mL）显著停止金黄色葡萄球菌的发展，铜绿假单胞菌和白色念珠菌生物膜分别为80.3±1.4、68.7±3.0和55.7±3.0％，分别。然而,对于100和200µg/ml的CuTiNCs，记录了64.63±3.5和89.82±4.3％的抗微生物污染潜力，分别；突出了它们对海洋微霉菌细胞的破坏作用，并通过SEM观察了它们的细胞外聚合物骨架的衰变。此外，CuTiNCs（100和200µg/ml）在2小时内通过减少微生物负荷（即，总平板计数，霉菌和酵母，家庭和农业废水中的总大肠杆菌和粪便链球菌）达到>50%。
结论：CuNPs和TiNPs在真菌功能化的CuTiNCs中提供的协同效率增强了其作为抗植物病原性的募集，抗生物膜,不同领域的防污剂和消毒剂。
