Abstract：
In order to develop a safe and effective broad-spectrum vaccine for foot-and-mouth disease (FMDV), here, we developed a recombinant FMD multiple-epitope trivalent vaccine based on three distinct topotypes of FMDV. Potency of the vaccine was evaluated by immune efficacy in pigs. The results showed that the vaccine with no less than 25 μg of antigen elicited FMDV serotype O specific antibodies and neutralization antibodies by primary-booster regime, and offered immune protection to pigs. More importantly, the vaccine elicited not only the same level of neutralization antibodies against the three distinct topotypes of FMDV, but also provided complete protection in pigs from the three corresponding virus challenge. None of the fully protected pigs were able to generate anti-3ABC antibodies throughout the experiment, which implied the vaccine can offer sterilizing immunity. The vaccine elicited lasting-long high-level antibodies and effectively protected pigs from virulent challenge within six months of immunization. Therefore, we consider that this vaccine may be used in the future for the prevention and control of FMD.
摘要：
为了研制出安全有效的口蹄疫广谱疫苗,在这里，我们基于三种不同的FMDV拓扑类型开发了重组FMD多表位三价疫苗.通过在猪中的免疫效力评价疫苗的效力。结果表明，抗原不低于25μg的疫苗通过一级加强方案引发FMDV血清型O特异性抗体和中和抗体，并为猪提供免疫保护。更重要的是,该疫苗不仅引发了针对FMDV三种不同拓扑类型的相同水平的中和抗体，但也提供了完全保护猪免受三种相应的病毒攻击。在整个实验过程中，完全受保护的猪都无法产生抗3ABC抗体，这意味着疫苗可以提供无菌免疫。该疫苗可引发持久的高水平抗体，并在免疫后六个月内有效保护猪免受毒力攻击。因此,我们认为这种疫苗将来可能用于FMD的预防和控制。
