PDF(Pubmed)
Abstract：
Brassica vegetables exhibit pronounced heterosis; nevertheless, investigations on fertility-related genes are scarce. The present study scrutinized a recessive genic male-sterile line, 7-3A, capable of generating a completely sterile population, holding significant promise for flowering Chinese cabbage breeding. By whole-genome resequencing of sterile and fertile plants, the male-sterile gene was confined to approximately 185 kb on chromosome A07, situated between markers C719 and NP10 in Brassica rapa var. Chiifu-401. Notably, substantial structural variation was identified within this region across diverse Brassica rapa reference genomes. Despite discernible expression level disparities of a homologous gene, Bnams4b, between male sterile and fertile plants, no sequence divergence was detected. Further elucidation is required to pinpoint a novel sterile gene within the candidate interval. This investigation contributes to the advancement of both the molecular-assisted breeding scheme for flowering Chinese cabbage and the comprehension of male sterility mechanisms.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-024-04005-7.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-024-04005-7.
摘要：
芸苔属蔬菜表现出明显的杂种优势；尽管如此，对生育相关基因的研究很少。本研究仔细检查了隐性基因雄性不育系，7-3A,能够产生完全不育的种群，对开花的大白菜育种有着重大的希望。通过对不育和可育植物的全基因组重测序，雄性不育基因被限制在甘蓝型油菜中位于标记C719和NP10之间的A07染色体上约185kb。Chiifu-401.值得注意的是,在不同的芸苔属参考基因组中，在该区域内发现了大量结构变异。尽管同源基因的表达水平存在明显差异，Bnams4b,在雄性不育和可育植物之间，没有检测到序列差异。需要进一步阐明以查明候选间隔内的新无菌基因。这项研究有助于提高大白菜的分子辅助育种方案和对雄性不育机制的理解。
■在线版本包含补充材料，可在10.1007/s13205-024-04005-7获得。
■在线版本包含补充材料，可在10.1007/s13205-024-04005-7获得。
